Anti-GPR139 Antibody | Alomone Labs AGR-069 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR139 Antibody

catalog :

AGR-069

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysates (lanes 2 and 4): - 1-2. Anti-GPR139 Antibody (#AGR-069), (1:800). 3-4. Anti-GPR139 Antibody, preincubated with GPR139 Blocking Peptide (BLP-GR069).

image 2 :

Expression of GPR139 in rat ventromedial hypothalamus (VMH). - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR139 Antibody (#AGR-069), (1:600), followed by goat anti-rabbit-AlexaFluor-488. A. GPR139 immunoreactivity (green) appears in tanycyte-like processes (arrows). B. Pre-incubation of the antibody with GPR139 Blocking Peptide (BLP-GR069), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third ventricle.

product information

CAT :

AGR-069

SKU :

AGR-069-CF_0.2 ml

Product Name :

Anti-GPR139 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q80UC8

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR069

Homology :

Rat, Human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RKSNFRLRGYSTGK, corresponding to amino acid residues 204 - 217 of mouse GPR139

Product Page - Scientific background :

G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs are a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.GPR139 is an orphan GPCRs for which amino acids L-tryptophan and L-phenylalanine, the adrenocorticotropic hormone (ACTH), and α- and β-melanocyte-stimulating hormone have been proposed as endogenous agonists3. GPR139 was found to be highly conserved across species, as the amino acid sequence of the human GPR139 protein is 96%, 92% and 70% identical to the mouse, chicken and zebrafish orthologs, respectively. According to mRNA expression levels GPR139 is mainly expressed in the central nervous system (CNS). GPR139 has been reported to activate several G protein pathways of which Gq/11 is the primary one4.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

G Protein-Coupled Receptor 139, PGR3, G(Q)-Coupled Orphan Receptor GPRg1, GPRG1

Short Description :

A Rabbit Polyclonal antibody to Parkin

Long Description :

Anti-GPR139 Antibody (#AGR-069) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR139 from rat, mouse and human samples.

Negative Control :

BLP-GR069

Positive Control :

Synonyms :

G Protein-Coupled Receptor 139, PGR3, G(Q)-Coupled Orphan Receptor GPRg1, GPRG1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR139

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200-1:800

more info or order :

Alomone Labs product webpage