antibody

Anti-GPR18 (extracellular) Antibody

AGR-068

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

western blot, immunohistochemistry

AGR-068-CF_0.2 ml

Anti-GPR18 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q8K1Z6

IFC IHC LCI WB

Rat Mouse

Rabbit

BLP-GR068

Rat - 13 out of 16 amino acid residues identical Not recommended for human samples

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)HNQLDLSNGSHPEEYK, corresponding to amino acid residues 7 - 22 of mouse GPR18

G protein coupled receptors (GPCRs) comprise a large superfamily of receptors characterized by a seven transmembrane domain structure and an ability to activate intracellular transducer G proteins. Over 800 GPCRs in five main families have been identified in eukaryotes on the basis of genomic sequence analysis 1-2. These receptors can be activated by hormones, neurotransmitters, odorants, light, or pheromones. GPCRs mediate the signaling of extracellular stimuli to intracellular responses via activation of heterotrimeric G proteins and their subsequent interaction with effector proteins. GPCRs have been implicated in a number of physiological functions including development of anxiety, behavior, homeostasis, cognition, appetite, and drug addiction 3. The GPCR family contains a diverse group of cell-surface mediators of signal transduction, its size exceeding other cell-surface protein receptors including the tyrosine kinase receptors, guanylyl cyclase receptors and ligand-gated ion channels 4. The murine homolog of GPR18 was first isolated in 1996 5. A potential agonist for this orphan receptor was proposed only in 2006 when N-arachidonylglycine (NAGly) was shown to increase calcium concentration and inhibit forskolin induced cAMP production in CHO cells in a pertussis sensitive manner 6. GPR18 shares low sequence homology with the cannabinoid receptors CB1R and CB2R (~13% and 8%) and moderate identity with the putative cannabinoid receptor GPR55 (21%). Nonetheless, it has been related to the endocannabinoid system since a range of endogenous, phytogenic and synthetic cannabinoids have shown to modulate GPR18 7. GPR18 was shown to be constitutively active in melanomas, acting as an apoptosis inhibitor 8, while playing a reverse role in macrophage apoptosis, that is, signaling for cell death9. Both of these roles were mediated via Gαi pathways. Work on alternate cannabinoid compounds as potential agonist showed differential activation of Gαq / Gαi signaling suggesting biased agonism in pathway selection10.

IFC IHC LCI WB

Alomone Labs

G-Protein Coupled Receptor 18, N-Arachidonyl Glycine Receptor, NAGly Receptor, GPCRW

A Rabbit Polyclonal antibody to GPR18 (extracellular)

Anti-GPR18 (extracellular) Antibody (#AGR-068) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR18 from rat and mouse samples. The antibody will not recognize GPR18 from human samples.

BLP-GR068

NA

G-Protein Coupled Receptor 18, N-Arachidonyl Glycine Receptor, NAGly Receptor, GPCRW

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

GPR18

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:1000

1:200