Anti-GPR31 (extracellular) Antibody | Alomone Labs AGR-064 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR31 (extracellular) Antibody

catalog :

AGR-064

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):

image 2 :

Western blot analysis of mouse P815 mast cell line lysate (lanes 1 and 3) and mouse J774 macrophage cell line lysate (lanes 2 and 4):

image 3 :

Expression of GPR31 in rat hippocampus.

product information

CAT :

AGR-064

SKU :

AGR-064-CF_0.2 ml

Product Name :

Anti-GPR31 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

F8VQN3

Applications :

IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR064

Homology :

Mouse - 16 out of 17 amino acid identical Rat - 14 out of 17 amino acid identical It won't recognize human GPR31

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CRTTQNSTE(S)PSFYPTG, corresponding to amino acid residues 153 - 169 of mouse GPR31

Product Page - Scientific background :

GPR31, also known as 12-HETER or HETER1, is a member of the G protein-coupled receptor (GPCR) superfamily.GPR31 was found to be a high affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE), a metabolite of arachidonic acid produced by the action of the enzyme 12-lipoxygenase (12-LOX), even though GPR31 is still formally considered as a Class A orphan GPCR receptor.1-2Interestingly, 12-HETE is also a ligand for other GPCRs like Thromboxane A2 receptor or ion channels like TRPV1, although at lower affinities.2GPR31 is expressed in various tissues, including the gastrointestinal tract, liver, brain and several types of immune cells.The involvement of GPR31 in various aspects of the inflammatory process have been intensely studied. GPR31plays a key role in hepatic ischemia-reperfusion injury. It is responsible for mediating the inflammatory response triggered by the accumulation of 12-HETE, produced by the enzyme ALOX12.3In addition, it was shown that GPR31 to plays a crucial role in the immune response by regulating the activity of intestinal CX3CR1+ cells. GPR31 activation promotes dendritic cell protrusions of intestinal CX3CR1+ cells, a mechanism is essential for maintaining intestinal homeostasis and could be pivotal in inflammatory conditions where the immune system's interaction with gut microbiota is disrupted.4, 6GPR31 has been implicated in cancer biology. Its activation can promote the migration and invasion of cancer cells, contributing to metastasis. Overexpression of GPR31 has been observed in certain types of cancer including pancreatic, prostate, and breast cancers.5, 6 In this context, it is interesting to note that GPR31 can also be activated by acidic extracellular conditions, a hallmark of the tumor microenvironment.7In brief, GPR31 receptor has an important role in the regulation of cell migration, cancer metastasis, and inflammation. Its role in these processes makes it a significant target for potential therapeutic interventions in cancer and inflammatory diseases.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

G Protein-Coupled Receptor 31, 12-(S)-HETE Acid Receptor, 12-HETER, GPR31/12-HETER

Short Description :

A Rabbit Polyclonal antibody to GPR31 (extracellular)

Long Description :

Anti-GPR31 (extracellular) Antibody (#AGR-064) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR31 from mouse and rat samples. The antibody will not recognize human GPR31.

Negative Control :

BLP-GR064

Positive Control :

Synonyms :

G Protein-Coupled Receptor 31, 12-(S)-HETE Acid Receptor, 12-HETER, GPR31/12-HETER

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR31

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage