Anti-GPR12 (extracellular) Antibody | Alomone Labs AGR-062 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR12 (extracellular) Antibody

catalog :

AGR-062

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1-2. Anti-GPR12 (extracellular) Antibody (#AGR-062), (1:200). 3-4. Anti-GPR12 (extracellular) Antibody, preincubated with GPR12 (extracellular) Blocking Peptide (BLP-GR062).

image 2 :

Expression of GPR12 in rat cortex. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR12 (extracellular) Antibody (#AGR-062), (1:300), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. Staining in the parietal cortex region, showed GPR12 immunoreactivity (red) in glial cell outlines (arrows). B. Pre-incubation of the antibody with GPR12 (extracellular) Blocking Peptide (BLP-GR062), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGR-062

SKU :

AGR-062-CF_0.2 ml

Product Name :

Anti-GPR12 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35412

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR062

Homology :

Rat - identical, Human - 12 out of 13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-term.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EDPKVNLSGLPRD, corresponding to amino acid residues 3 - 15 of mouse GPR12

Product Page - Scientific background :

G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs is a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.GPR12 is an orphan G protein-coupled receptor related phylogenetically, functionally, and pharmacologically with the cannabinoid system. It considered as a "constitutively active receptor" since it greatly stimulates adenylate cyclase in the absence of ligand-receptor interaction. GPR12 seems to couple Gαs as well as Gαi proteins since it stimulates baseline cAMP-dependent signaling by activating Gαs proteins, and also inhibits forskolin activated signaling through Gαi interaction3.GPR12 expressed in the pituitary gland, in neurons in the frontal cortex, piriform cortex, thalamus, hypothalamus, hippocampus, amygdala, and olfactory bulb. It was found that GPR12 plays a significant role in energy balance. GPR12 knock out mice exhibit increase body weight and body fat mass. In addition, overexpression of GPR12 leads to cancer cells migration and invasion via phosphorylation of Keratin 84.Furthermore, GPR12 has been identified as an inverse agonist of the phytocannabinoid cannabidiol (CBD)4.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

G-Protein Coupled Receptor 12, GPCR21

Short Description :

A Rabbit Polyclonal antibody to SORCS2

Long Description :

Anti-GPR12 (extracellular) Antibody (#AGR-062) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR12 from rat, mouse and human samples.

Negative Control :

BLP-GR062

Positive Control :

Synonyms :

G-Protein Coupled Receptor 12, GPCR21

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR12

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage