Anti-GPR50 Antibody | Alomone Labs AGR-061 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR50 Antibody

catalog :

AGR-061

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-GPR50 Antibody (#AGR-061), (1:200).3-4. Anti-GPR50 Antibody, preincubated with GPR50 Blocking Peptide (BLP-GR061).

product information

CAT :

AGR-061

SKU :

AGR-061-CF_0.2 ml

Product Name :

Anti-GPR50 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88495

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR061

Homology :

Rat - identical, Human - 14 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ESFRREYWTIFHAMR, corresponding to amino acid residues 307 - 321 of mouse GPR50

Product Page - Scientific background :

G protein-coupled receptor 50 (GPR50), previously known as the melatonin-related receptor, share 45% sequence identity to melatonin receptors MT1 and MT2 but it does not bind melatonin or any other known ligand and therefore remains an orphan receptor without a clear function1,2.GPR50 belongs to the G protein coupled receptors (GPCRs) family, a large group of receptors characterized by seven trans membrane helices that transmit a signal from the outside cellular surface into the inside of the cell through conformational changes. GPCRs mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants, and so have great potential as therapeutic targets for a broad spectrum of diseases1.GPR50 was identified as a risk gene for bipolar disorder and major depression and it was shown to have a role in neural development and sterol metabolism, it was also shown to be related to mood disorders, adaptive thermogenesis and torpor response (the body's response to starvation)2. In addition, it was shown to have a dramatic effect on neurite outgrowth, supporting a likely role in neural development and it was also found to be expressed in various types of cancer3.GPR50 is expressed in the pituitary, hypothalamus, cortex, midbrain, pons, amygdala, and in several brainstem nuclei3,4. Due to its involvement in a wide range of mental conditions and disease, GPR50 is a major target for research and drug development.

Applications may also work in :

Supplier :

Alomone Labs

Target :

G Protein-Coupled Receptor 50, Melatonin-Related Receptor

Short Description :

A Rabbit Polyclonal antibody to GPR50

Long Description :

Anti-GPR50 Antibody (#AGR-061) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot applications. It has been designed to recognize GPR50 from mouse, rat and human samples.

Negative Control :

BLP-GR061

Positive Control :

Synonyms :

G Protein-Coupled Receptor 50, Melatonin-Related Receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR50

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage