Anti-GPR174 (extracellular)-FITC Antibody | Alomone Labs AGR-060-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR174 (extracellular)-FITC Antibody

catalog :

AGR-060-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of GPR174 by direct flow cytometry in live intact mouse TK-1 T-cell lymphoma cells: Cell surface detection of GPR174 by direct flow cytometry in live intact mouse TK-1 T-cell lymphoma cells: Black line: Cells. Red line: Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F). Green line: Cells + Anti-GPR174 (extracellular)-FITC Antibody (#AGR-060-F), (5µg).

product information

CAT :

AGR-060-F

SKU :

AGR-060-F_15 mcl

Product Name :

Anti-GPR174 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9BXC1

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR060

Homology :

Human - 15/16 amino acid residues identical; mouse, rat - 13/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop.

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DTSGNRTK(S)FVDLPTR, corresponding to amino acid residues 160 - 175 of human GPR174

Product Page - Scientific background :

GPR174 (LysoPS, LYPSR3), is one of three lysophosphatidyl serine receptors, belonging to the superfamily of G-protein coupled receptors. Lysophospholipids are a diverse group of small, membrane-derived phospholipids that act as extracellular signals1.Activation of GPR174 leads to an increase in cAMP cellular levels, leading to cellular morphological changes, cell to cell adhesion, and delays in cell proliferation2.GPR174 is reported to be a genetic risk locus for Graves disease, an autoimmune disorder that causes hyperthyroidism3 and as a negative regulator of T-cells activation4.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79

Short Description :

A Rabbit Polyclonal Antibody to GPR174 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-GPR174 (extracellular) Antibody (#AGR-060) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR174 from rat, mouse and human samples. Anti-GPR174 (extracellular)-FITC Antibody (#AGR-060-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize GPR174 from rat, mouse and human samples.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR174

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGR-060), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage