Anti-GPR52 Antibody | Alomone Labs AGR-058 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR52 Antibody

catalog :

AGR-058

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4): - 1, 2. Anti-GPR52 Antibody (#AGR-058), (1:200). 3, 4. Anti-GPR52 Antibody, preincubated with GPR52 Blocking Peptide (#BLP-GR058) (#BLP-GR058).

image 2 :

Expression of GPR52 in rat substantia nigra - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR52 Antibody (#AGR-058), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GPR52 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with GPR52 Blocking Peptide (#BLP-GR058), suppresses staining. Cell nuclei are stained with DAPI (blue). SNR = substantia nigra pars reticulata.

product information

CAT :

AGR-058

SKU :

AGR-058-CF_0.2 ml

Product Name :

Anti-GPR52 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P0C5J4

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR058

Homology :

Rat - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)DRRARFPSHEVEASR, corresponding to amino acid residues 239 - 253 of mouse GPR52

Product Page - Scientific background :

G-Protein Coupled Receptor 52 (GPR52) is a conserved class-A orphan receptor, a member of the of the GPCR superfamily. The native ligand for GPR52 is unknown but the receptor has a high level of basal activity not related to ligand binding. Structure analysis shows that GPR52 extracellular loop 2 occupies the orthosteric binding pocket and operates as a built-in agonist, enabling an intrinsically high level of basal activity. The receptor also features a side pocket for ligand binding3.Tissue distribution analysis of human, mouse, and rat indicates that GPR52 is expressed exclusively in the brain, especially in striatum. In the human brain, GPR52 expression profile overlaps with the distribution of D1 and D2 dopamine receptors which are associated with its function1.GPR52 inhibits dopamine D2 receptor signaling and activates dopamine D1/NMDA receptors via intracellular cAMP accumulation2.GPR52 is involved in the manifestation of schizophrenia, Huntington's disease, cognitive impairment and hyperactivity and several other psychiatric disorders and hence it represents a promising therapeutic target. Agonists may potentially act as a novel class of antipsychotics1,2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

G-Protein Coupled Receptor 52

Short Description :

A Rabbit Polyclonal Antibody to GPR52

Long Description :

Anti-GPR52 Antibody (#AGR-058) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry. It has been designed to recognize GPR52 from human, mouse, and rat samples.

Negative Control :

BLP-GR058

Positive Control :

Synonyms :

G-Protein Coupled Receptor 52

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR52

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage