Anti-GPR97 (ADGRG3)(extracellular) Antibody | Alomone Labs AGR-057 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR97 (ADGRG3)(extracellular) Antibody

catalog :

AGR-057

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057), (1:200). 3-4. Anti-GPR97 (ADGRG3)(extracellular) Antibody, preincubated with GPR97 (ADGRG3)(extracellular) Blocking Peptide (BLP-GR057).

image 2 :

Expression of GPR97 in rat spinal cord. - Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn of the spinal cord region, showed immunoreactivity of GPR97 (green) in neuronal outlines (arrows). B. Pre-incubation of the antibody with GPR97 (ADGRG3)(extracellular) Blocking Peptide (BLP-GR057), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGR-057

SKU :

AGR-057-CF_0.2 ml

Product Name :

Anti-GPR97 (ADGRG3)(extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q86Y34

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR057

Homology :

Mouse, rat - 12 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-term

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CNVENLQRYWLNYE, corresponding to amino acid residues 62 - 75 of human ADGRG3

Product Page - Scientific background :

Adhesion G protein-coupled receptors (aGPCRs) are one of the five main families in the GPCR superfamily. They act as a molecular switch that regulate many physiological processes, including brain development, ion-water homeostasis, inflammation and cell-fate determination1.One member of the aGPCRs family is the orphan receptor GPR97 (also known as ADGRG3). GPR97 consist of seven-transmembrane domain and a GPCR-Autoproteolysis INducing (GAIN) domain. Auto-cleavage via the GAIN domain produces two subunits: the α-subunit (N-terminal fragment (NTF)) and the β-subunit (C-terminal fragment) that remain non-covalently associated at the cell surface2. GPR97 is mainly expressed in immune cells such as neutrophils, eosinophils, and mast cells. Activation of GPR97 results in reduction in cAMP levels in combination with an increase in downstream effectors of Gβγ, such as SRE and NF-κB3. GPR97 was found to regulate B-cell development in mice and antimicrobial activity in human granulocytes3. In addition, studies have shown that GPR97 is involved in macrophage inflammation in high fat diet-induced obesity in mice4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97

Short Description :

A Rabbit Polyclonal antibody to GPR97 (ADGRG3) (extracellular)

Long Description :

Anti-GPR97 (ADGRG3)(extracellular) Antibody (#AGR-057) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR97 from rat, mouse and human samples.

Negative Control :

BLP-GR057

Positive Control :

Synonyms :

Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADGRG3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage