antibody

Anti-GPR34 (extracellular) Antibody

AGR-055

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

AGR-055-CF_0.2 ml

Anti-GPR34 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q9R1K6

IFC WB

Human Rat Mouse

Rabbit

BLP-GR055

Human, rat - 12/13 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

2nd extracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

CFHYRDRHNAKGE, corresponding to amino acid residues 197 - 209 of mouse GPR34

The G-protein-coupled receptor 34 (GPR34) is currently classified as an orphan receptor of the P2Y12-like receptor group. The receptor belongs to the super family of G-protein coupled receptors (GPCRs)1.The N-terminus of human GPR34 has three putative N-glycosylation sites (NXS/T), and the C-terminus has a cysteine residue which is palmitoylated in many GPCRs. GPR34 contains several putative phosphorylation sites primed by a serine or threonine in the C-terminus2.GPR34 is highly expressed in immune cells such as mast cells, macrophages, microglia, and immune system-derived cell lines (HL-60, K562, WEHI-3B, RAW 264.7, P815). Studies shows that GPR34 function is required to arrest microglia in the M0 homeostatic non-phagocytic phenotype and downregulation of GPR34 promotes the neurodegenerative microglia phenotype2,3,4.GPR34 is linked to several human diseases including immunological diseases and cancer. Most known effects of GPR34 are immune function-related. For example, GPR34 is significantly down-regulated in Mycobacterium tuberculosis-infected macrophages; GPR34 mRNA is significantly lower in the cerebrospinal fluid from multiple sclerosis patients; GPR34 is significantly upregulated in the striatum and cortex of patients with Parkinson's disease but downregulated in substantia nigra. elevated GPR34 also plays a role in the progression of some tumors, such as human gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and colorectal cancer1-3.

IFC WB

Alomone Labs

G-protein coupled receptor 34, LYPSR1

A Rabbit Polyclonal Antibody to GPR34

Anti-GPR34 (extracellular) Antibody (#AGR-055) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize GPR34 from human, mouse, and rat samples.

BLP-GR055

NA

G-protein coupled receptor 34, LYPSR1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

GPR34

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:200