Anti-GPR171 (extracellular) Antibody | Alomone Labs AGR-054 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR171 (extracellular) Antibody

catalog :

AGR-054

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1, 2. Anti-GPR171 (extracellular) Antibody (#AGR-054), (1:200). 3, 4. Anti-GPR171 (extracellular) Antibody, preincubated with GPR171 (extracellular) Blocking Peptide (#BLP-GR054).

product information

CAT :

AGR-054

SKU :

AGR-054-CF_0.2 ml

Product Name :

Anti-GPR171 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BG55

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR054

Homology :

Mouse, human - 14/15 amino acid residues identical; rat - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

Peptide (C)KDIKEKSNVG(S)MEFK, corresponding to amino acid residues 155 - 169 of mouse GPR171.

Product Page - Scientific background :

G protein-coupled receptor 171 (GPR171) is an orphan G-protein coupled receptor (GPCR) activated by BigLEN; one of the most abundant neuropeptides in the brain that is derived from ProSAAS1. GPR171 contains seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. Its detailed structure and ligand binding domain structure was predicted by homology modeling based on its similarity to P2Y12 receptor2,3.BigLEN-GPR171 neuropeptide-receptor system is expressed in the brain, at the basolateral amygdala (BLA) and the periaqueductal gray brain area4-6.This system plays an important role in regulating feeding-related behaviors, food intake and body weight regulation. It is also involved in anxiety behavior and various psychiatric disorders4,5.Furthermore, BigLEN-GPR171 are expressed in GABAergic neurons within the periaqueductal gray, a key brain area involved in pain modulation and opioid functions6.GPR171 is a potential target for drug development in obesity and weight problems, anxiety, various psychiatric disorders and pain.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963

Short Description :

A Rabbit Polyclonal Antibody to GPR171

Long Description :

Anti-GPR171 (extracellular) Antibody (#AGR-054) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize GPR171 from human, rat, and mouse samples.

Negative Control :

BLP-GR054

Positive Control :

Synonyms :

G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR171

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage