Anti-GPR83 (extracellular) Antibody | Alomone Labs AGR-053 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR83 (extracellular) Antibody

catalog :

AGR-053

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Ghosh B, Zhang C, Ziemba K, Fletcher A, Yurek D, Smith G. Partial Reconstruction of the Nigrostriatal Circuit along a Preformed Molecular Guidance Pathway. Mol Ther Methods Clin Dev. 2019;14:217-227 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1, 2. Anti-GPR83 (extracellular) Antibody (#AGR-053), (1:400). 3, 4. Anti-GPR83 (extracellular) Antibody, preincubated with GPR83 (extracellular) Blocking Peptide (#BLP-GR053).

image 2 :

Expression of GPR83 in mouse hippocampus and cortex - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR83 (extracellular) Antibody (#AGR-053), (1:3000), followed by goat anti-rabbit-AlexaFluor-488. A. GPR83 staining (green) in hippocampal dentate gyrus is detected in hilar interneurons (vertical arrows) and around cells of the granule layer (horizontal arrows). H = hilus of dentate gyrus. G = granule layer. B. GPR83 immunoreactivity (green) in parietal cortex is observed in neurons of the pyramidal layer (vertical arrows) and in their apical dendrites (horizontal arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGR-053

SKU :

AGR-053-CF_0.2 ml

Product Name :

Anti-GPR83 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30731

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR053

Homology :

Human, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RRYGAESQNPTVK, corresponding to amino acid residues 59 - 71 of mouse GPR83

Product Page - Scientific background :

GPR83 (JP05, GIR, GPR72) is a G-protein coupled receptor (GPCR) highly expressed in the brain, spleen and thymus, suggesting that this receptor may be targeted to treat neurological and immune disorders1.GPCR is a family of receptors responsible for initiation of a myriad of intracellular signaling cascades, these receptors are characterized by an extracellular N-terminus, intracellular C-terminal tail, and seven membrane-spanning α-helices (TM-1 to TM-7)2.In the brain GPR83 is expressed in the hippocampus, amygdala, prefrontal cortex, and in various hypothalamic nuclei where it may play a significant role in learning memory, reward, emotional behaviors, and stress regulation3. GPR83 is also expressed in regulatory T cells (Tregs) and it could potentially be used as a specific marker used to identify natural Tregs4.GPR83 was considered as an "orphan" GPCR. Recently, PEN, a neuropeptide was identified as its endogenous ligand1.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR

Short Description :

A Rabbit Polyclonal Antibody to GPR83

Long Description :

Anti-GPR83 (extracellular) Antibody (#AGR-053) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPR83 from rat, mouse, and human samples.

Negative Control :

BLP-GR053

Positive Control :

Synonyms :

G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR83

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:3000

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage