Anti-GPR84 (extracellular) Antibody | Alomone Labs AGR-052 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR84 (extracellular) Antibody

catalog :

AGR-052

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat spleen membranes (lanes 3 and 6): - 1-3. Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200).4-6. Anti-GPR84 (extracellular) Antibody, preincubated with GPR84 (extracellular) Blocking Peptide (#BLP-GR052).

image 2 :

Expression of GPR84 in mouse fornix in a kainic acid-induced model of temporal lobe epilepsy - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. In untreated mouse sections GPR84 immunoreactivity (green) is detected in astrocyte profiles (arrows). B. GPR84 staining (green) in mouse sections obtained four days post kainate-induced seizures, shows an increase in number and size of astrocyte profiles stained for GPR84 (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGR-052

SKU :

AGR-052-CF_0.2 ml

Product Name :

Anti-GPR84 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9NQS5

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR052

Homology :

Human - 14/15 amino acid residues identical; mouse, rat - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84

Product Page - Scientific background :

GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive Gi/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer1-3.GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production1-3.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

Short Description :

A Rabbit Polyclonal Antibody to GPR84

Long Description :

Anti-GPR84 (extracellular) Antibody (#AGR-052) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR84 from human, rat, and mouse samples.

Negative Control :

BLP-GR052

Positive Control :

Synonyms :

G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR84

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage