Anti-GPR37L1 (extracellular) Antibody | Alomone Labs AGR-050 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR37L1 (extracellular) Antibody

catalog :

AGR-050

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:200). 3-4. Anti-GPR37L1 (extracellular) Antibody, preincubated with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050).

image 2 :

Expression of GPR37L1 in rat cerebellum. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GPR37L1 immunoreactivity (green) appeared around Purkinje cell soma (arrows) and in the molecular layer (M). B. Pre-incubation of the antibody with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGR-050

SKU :

AGR-050-CF_0.2 ml

Product Name :

Anti-GPR37L1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QYC5

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR050

Homology :

Mouse - identical; human - 14 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EYPKPIHPAGLQPTK, corresponding to amino acid residues 66 - 80 of rat GPR37L1

Product Page - Scientific background :

G prtoein coupled receptor 37L1 (GPR37L1), also known as Endothelin B Receptor-Like Protein 2, is an orphan receptor (a receptor without an assigned ligand yet) that shares structural homology with the endothelin receptors family of GPCRs. 1GPR37L1 is a 481 amino acid GPCR with 68% similarity and 48% identity to GPR37, another orphan endothelin receptor like GPR. In addition to the seven trans- membrane helixes typical for GPCRs, it has a cysteine cluster at the end of helix 8. 2GPR37L1 has been shown to bind the neuroprotective and glioprotective factor prosaposin (PSAP), although later studies did not confirm prosaposin as a functional ligand. 3GPR37L1 is highly expressed in the central nervous system, with some expression in the spinal cord, placenta, liver, stomach, and testis. 4,5GPR37L1 signaling pathway was shown to have an effect on motor activity, blood pressure regulation, cellular development and protection from cytotoxic agents. Deletion of the receptor caused marked developmental changes in the cerebellum, and hence the discovery of the native ligand for this receptor and studying the signaling pathway that is induced by it, is of high medicinal importance 1.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

G-Protein Coupled Receptor 37-Like 1, Endothelin B Receptor-Like Protein 2, ETBRLP2, Prosaposin Receptor GPR37L1

Short Description :

A Rabbit Polyclonal Antibody to GPR37L1 (extracellular)

Long Description :

Anti-GPR37L1 (extracellular) Antibody (#AGR-050) is a highly specific antibody directed against an extracellular epitope of the rat GPR37L1. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR37L1 from mouse, rat and human samples.

Negative Control :

BLP-GR050

Positive Control :

Synonyms :

G-Protein Coupled Receptor 37-Like 1, Endothelin B Receptor-Like Protein 2, ETBRLP2, Prosaposin Receptor GPR37L1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR37L1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage