Anti-GPR17 Antibody | Alomone Labs AGR-048 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR17 Antibody

catalog :

AGR-048

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1,2. Anti-GPR17 Antibody (#AGR-048), (1:200).3,4. Anti-GPR17 Antibody, preincubated with GPR17 Blocking Peptide (#BLP-GR048).

image 2 :

Expression of GPR17 in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR17 Antibody (#AGR-048), (1:300), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. GPR17 immunoreactivity (red) appears in cortical cells (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGR-048

SKU :

AGR-048-CF_0.2 ml

Product Name :

Anti-GPR17 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q09QM4

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR048

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 3rd loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RQGPRIEKHLKNK, corresponding to amino acid residues 220-232 of rat GPR17

Product Page - Scientific background :

GPR17 is an orphan receptor that can be double-activated by uracil and cysteinyl-leukotriene (CysLT). It is phylogenetically related to both purinergic P2Y and CysLT receptors. GPR17 belongs to the superfamily of G-protein coupled receptors (GPCRs)4.GPR17 expression is highly detected in the brain, kidney, and heart. In the brain, GPR17 is mainly distributed in white matter, gray matter, subependymal zone, and corpus callosum1.Studies have shown that GPR17 receptor plays an important role in spinal cord injury, myelin sheath injury, cerebral ischemic injury, where its expression is extremely upregulated1,2.GPR17 activation promotes oligodendrocyte differentiation and progression toward mature myelin-forming cells. Thus, GPR17 modulators may be used as therapeutic tools to treat pathologies associated with myelin repair in CNS demyelinating diseases3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 17, Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT Receptor, P2Y-like receptor

Short Description :

A Rabbit Polyclonal Antibody to GPR17 Receptor

Long Description :

Anti-GPR17 Antibody (#AGR-048) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR17 from rat and mouse samples. It is unlikely to recognize the protein from human samples.

Negative Control :

BLP-GR048

Positive Control :

Synonyms :

G-protein coupled receptor 17, Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT Receptor, P2Y-like receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR17

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage