Anti-GPR56 (extracellular)-FITC Antibody | Alomone Labs AGR-047-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR56 (extracellular)-FITC Antibody

catalog :

AGR-047-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of GPR56 in live intact mouse BV-2 microglia cells: Cell surface detection of GPR56 in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-GPR56 (extracellular)-FITC Antibody (#AGR-047-F), (2.5 µg).

product information

CAT :

AGR-047-F

SKU :

AGR-047-F_50 mcl

Product Name :

Anti-GPR56 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8K3V3

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR047

Homology :

Mouse - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RHTGRLHLRYGKND, corresponding to amino acid residues 86-109 of rat GPR56

Product Page - Scientific background :

GPR56 (ADGRG1) is a member of the adhesion G-protein coupled receptor (GPCR) family, responsible for the regulation of cell adhesion, migration, polarity, and guidance. GPR56 levels are increased in many cancer types and seems to be relevant for tumor cell migration and invasion1,2. GPR56 structure is characterized by a long extracellular domain that is involved in cell-to-cell and cell-to-extracellular matrix interactions. The receptor contains a GPS motif, responsible for cleaving the protein. Seven different glycosylation sites along its N-terminal fragment are present1-3.Expression of Gpr56 mRNA can be found in neuronal progenitor cells of the cerebral cortical ventricular and subventricular zones during periods of neurogenesis. Studies show that GPR56 protein knockdown promotes mesenchymal differentiation of glioma stem-like initiating cells, accompanied by increased radioresistance in vitro and in vivo1-3.Mutations in GPR56 may cause bilateral frontoparietal polymicrogyria, a cobblestone-like cortical malformation, characterized by overmigrating neurons and the formation of neuronal ectopias on the surface of the brain2,3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1

Short Description :

A Rabbit Polyclonal Antibody to GPR56 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-GPR56 (extracellular) Antibody (#AGR-047) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect flow cytometry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize ADGRG1 from rat, mouse, and human samples. The antibody recognizes the N-terminal fragment (GPR56-NT) that can be secreted to the media. Anti-GPR56 (extracellular)-FITC Antibody (#AGR-047-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADGRG1

AB Specifictiy :

This antibody recognizes the N-terminal fragment (GPR56-NT), that can be secreted to the media.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGR-047), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage