Anti-Human GPR132/G2A (extracellular)-FITC Antibody | Alomone Labs AGR-046-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human GPR132/G2A (extracellular)-FITC Antibody

catalog :

AGR-046-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of GPR132 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells: Cell surface detection of GPR132 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-Human GPR132/G2A (extracellular)-FITC Antibody (#AGR-046-F), (2.5µg).

product information

CAT :

AGR-046-F

SKU :

AGR-046-F_50 mcl

Product Name :

Anti-Human GPR132/G2A (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9UNW8

Applications :

FC LCI

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-GR046

Homology :

Human only

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EDKET(S)FDMLQMDSR, corresponding to amino acid residues 181 - 195 of human GPR132

Product Page - Scientific background :

G-protein coupled receptor 132, (GPR132, G2A) is classified as a member of the proton sensing G-protein coupled receptor (GPCR) subfamily. Like other members of this subfamily, i.e. GPR4, OGR1 (GPR68), and TDAG8 (GPR65), G2A is expressed on the cell surface membrane and contains seven transmembrane domains, similar to serpentine receptors. GPR132 senses changes in extracellular pH, and activation of this proton-sensing receptor by acidosis transduces multiple downstream G protein signaling pathways1.GPR132 is expressed in a broad range of immunoregulatory cell types, including macrophages, dendritic cells, neutrophils, mast cells, T and B lymphocytes. It mediates immune cell migration as well as phagocytosis and regulates autoimmunity and lymphocyte homeostasis2.G2A also plays a role in tumor suppression: the tumor microenvironment is acidic due to glycolytic cancer cell metabolism, hypoxia, and deficient blood perfusion and hence, G2A and other proton sensing receptors may be involved in regulation of cancer cell metastasis and proliferation. Small molecule modulators of the pH-sensing GPCRs are being actively developed and evaluated. Research on pH-sensing GPCRs provides important insights into the molecular interaction between the tumor and its acidic microenvironment and may identify new targets for cancer therapy3.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

G protein-coupled receptor 132, G2 accumulation protein, G2A

Short Description :

A Rabbit Polyclonal Antibody to Human GPR132 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-Human GPR132/G2A (extracellular) Antibody (#AGR-046) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR132 from human samples only. The antibody will not recognize GPR132 from mouse and rat samples. Anti-Human GPR132/G2A (extracellular)-FITC Antibody (#AGR-046-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize GPR132 from human samples only. The antibody will not recognize GPR132 from mouse and rat samples.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

G protein-coupled receptor 132, G2 accumulation protein, G2A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR132

AB Specifictiy :

The antibody will not recognize rat or mouse samples.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGR-046), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage