antibody

Anti-GPR39 (extracellular) Antibody

AGR-045

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

AGR-045-CF_0.2 ml

Anti-GPR39 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q5U431

IFC LCI WB

Human Rat Mouse

Rabbit

BLP-GR045

Human - 11/12 amino acid residues identical; rat - 9/12 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

3rd extracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)KPKHDWTRTYFR, corresponding to amino acid residues 309-320 of mouse GPR39

GPR39 (G-protein coupled receptor 39) is member of the rhodopsin-like family of 7-transmembrane containing GPCRs and is closely related to the ghrelin/neurotensin receptor subfamily.The human GPR39 gene encodes the full-length receptor (GPR39-1a). GPR39 is expressed in various tissues including adipose tissue, heart and thyroid but is most abundant in the pancreas, GI tract, liver and kidney. The GPR39 receptor is comprised of an extracellular N-terminus, seven membrane spanning loops, and an intracellular C-terminus. It also contains four cysteine residues in its extracellular domains. These have been shown to form two disulfide bridges which are conserved within all vertebrate GPR39 sequences with the exception that the first bridge is absent from the fish receptors.Although GPR39 has a high degree of constitutive activity mediated through Gαq and Gα12/13 pathways, it has been discovered recently that Zn2+ serves as a ligand capable of activating the receptor. Following Zinc activation, both pathways are significantly enhanced and signaling occurs through the Gαs pathway as well.GPR39 has been implicated in several pathologies: Disruption of the GPR39 gene is associated with impaired insulin secretion. GPR39 knockout mice exhibit decreased insulin levels and lower levels of insulin promoting factor-1 and HNF-1α in pancreatic islets. GPR39 knockout mice also exhibit lower levels of CREB and BDNF proteins that are important in neuronal plasticity and antidepressant response thus leading to depressive-like behaviors in animals.

IFC LCI WB

Alomone Labs

G-protein coupled receptor 39, Zinc receptor GPR39

A Rabbit Polyclonal Antibody to GPR39

Anti-GPR39 (extracellular) Antibody (#AGR-045) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize GPR39 from human, mouse, and rat samples.

BLP-GR045

NA

G-protein coupled receptor 39, Zinc receptor GPR39

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

GPR39

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:200