Anti-GPR68 (OGR1) (extracellular) Antibody | Alomone Labs AGR-042 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR68 (OGR1) (extracellular) Antibody

catalog :

AGR-042

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 2

Reference
He X, Feng S, Hawkins C, Lawley L, Fan W, Xu Y, et al. G protein-coupled receptor 68 increases the number of B lymphocytes. Am J Blood Res. 2020;10:15-21 pubmed
Bhave S, Gade A, Kang M, Hauser K, Dewey W, Akbarali H. Connexin-purinergic signaling in enteric glia mediates the prolonged effect of morphine on constipation. FASEB J. 2017;31:2649-2660 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney lysates (lanes 1 and 3) and mouse kidney membranes (lanes 2 and 4): - 1,2. Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042), (1:200).3,4. Anti-GPR68 (OGR1) (extracellular) Antibody, preincubated with GPR68/OGR1 (extracellular) Blocking Peptide (#BLP-GR042).

product information

CAT :

AGR-042

SKU :

AGR-042-CF_0.2 ml

Product Name :

Anti-GPR68 (OGR1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BFQ3

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR042

Homology :

Rat, mouse, human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68

Product Page - Scientific background :

All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54%) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Short Description :

A Rabbit Polyclonal Antibody to GPR68 (OGR1)

Long Description :

Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042) is a highly specific antibody directed against an epitope of the mouse ovarian cancer G-protein coupled receptor 1. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR68 from rat, mouse, and human samples.

Negative Control :

BLP-GR042

Positive Control :

Synonyms :

Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR68

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage