Anti-GPR68 (OGR1) (extracellular)-APC Antibody | Alomone Labs AGR-042-APC product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR68 (OGR1) (extracellular)-APC Antibody

catalog :

AGR-042-APC

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of GPR68 by direct flow cytometry in live intact mouse J774 macrophage cells:

image 2 :

Cell surface detection of GPR68 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:

image 3 :

Multicolor flow cytometry with GPR68 and GPR132

product information

CAT :

AGR-042-APC

SKU :

AGR-042-APC_50 mcl

Product Name :

Anti-GPR68 (OGR1) (extracellular)-APC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BFQ3

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR042

Homology :

Rat, mouse, human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop

Label :

Allophycocyanin (APC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68

Product Page - Scientific background :

All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54%) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor, AI2A6

Short Description :

A Rabbit Polyclonal Antibody to GPR68 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)

Long Description :

Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize GPR68 from mouse, rat and human samples. Anti-GPR68 (OGR1) (extracellular)-APC Antibody (#AGR-042-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-APC

Positive Control :

Synonyms :

Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor, AI2A6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR68

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGR-042), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage