Anti-GPR132 (extracellular) Antibody | Alomone Labs AGR-036 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR132 (extracellular) Antibody

catalog :

AGR-036

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat dorsal root ganglia lysate: - 1. Anti-GPR132 (extracellular) Antibody (#AGR-036), (1:200). 2. Anti-GPR132 (extracellular) Antibody, preincubated with GPR132 (extracellular) Blocking Peptide (BLP-GR036).

product information

CAT :

AGR-036

SKU :

AGR-036-CF_0.2 ml

Product Name :

Anti-GPR132 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Z282

Applications :

IFC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR036

Homology :

Rat - 11 out of 15 amino acid residues identical It won't recognize human GPR132

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CFEPLRMNSKIAGYH, corresponding to amino acid residues 184 - 198 of mouse GPR132

Product Page - Scientific background :

G-protein coupled receptor 132, (GPR132, G2A) is classified as a member of the proton sensing G-protein coupled receptor (GPCR) subfamily. Like other members of this subfamily, i.e. GPR4, OGR1 (GPR68), and TDAG8 (GPR65), G2A is expressed on the cell surface membrane and contains seven transmembrane domains, similar to serpentine receptors. GPR132 senses changes in extracellular pH, and activation of this proton-sensing receptor by acidosis transduces multiple downstream G protein signaling pathways1.GPR132 is expressed in a broad range of immunoregulatory cell types, including macrophages, dendritic cells, neutrophils, mast cells, T and B lymphocytes. It mediates immune cell migration as well as phagocytosis and regulates autoimmunity and lymphocyte homeostasis2.G2A also plays a role in tumor suppression: the tumor microenvironment is acidic due to glycolytic cancer cell metabolism, hypoxia, and deficient blood perfusion and hence, G2A and other proton sensing receptors may be involved in regulation of cancer cell metastasis and proliferation. Small molecule modulators of the pH-sensing GPCRs are being actively developed and evaluated. Research on pH-sensing GPCRs provides important insights into the molecular interaction between the tumor and its acidic microenvironment and may identify new targets for cancer therapy3.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A

Short Description :

A Rabbit Polyclonal antibody to GPR132 (extracellular)

Long Description :

Anti-GPR132 (extracellular) Antibody (#AGR-036) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize GPR132 from mouse and rat samples. The antibody will not recognize GPR132 from human samples.

Negative Control :

BLP-GR036

Positive Control :

Synonyms :

G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR132

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage