Anti-QRFPR/GPR103 (extracellular) Antibody | Alomone Labs AGR-033 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-QRFPR/GPR103 (extracellular) Antibody

catalog :

AGR-033

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3), mouse brain membranes (lanes 2 and 4) and human LNCaP cell lysate (lanes 3 and 6): - 1-3. Anti-QRFPR/GPR103 (extracellular) Antibody (#AGR-033), (1:500).4-6. Anti-QRFPR/GPR103 (extracellular) Antibody, preincubated with QRFPR/GPR103 (extracellular) Blocking Peptide (#BLP-GR033).

product information

CAT :

AGR-033

SKU :

AGR-033-CF_0.2 ml

Product Name :

Anti-QRFPR/GPR103 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P83858

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR033

Homology :

Mouse - identical; human - 13/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HNLTREQFIHRYGLR, corresponding to amino acid residues 18-32 of rat QRFP receptor

Product Page - Scientific background :

QRFP is a 26-amino acid peptide (QRFP-26) that, widely distributed among vertebrates, is a member of the RF amide peptide family harboring the arginine-phenylalanine-amide (Arg-Phe-NH2) sequence motif in their carboxyl terminal. It is the cognate ligand of the human orphan receptor GPR103, a G protein-coupled receptor. Like other members of the super family, QRFP receptor has an extracellular N-terminal tail and intracellular C-terminus.Its QRFP N-terminus binds with high affinity to GPR103 and has the efficacy to inhibit cAMP synthesis. QRFP has been shown to affect locomotor activity, energy homeostasis, reproduction, bone formation, blood pressure, hormone regulation, nociception, and appetite stimulation.QRFP expressing neurons have shown to be expressed in various hypothalamic nuclei including the ventromedial hypothalamic nucleus, the lateral hypothalamic area, retrochiasmatic area and the arcuate nucleus1,2. The receptor is detected in the CNS and in peripheral tissues, notably in bone, heart, kidney, pancreas and testis.Increasing data suggest the ligand/receptor system plays an important role in the regulation of food intake and glucose homeostasis1.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27

Short Description :

A Rabbit Polyclonal Antibody to QRFP Receptor

Long Description :

Anti-QRFPR/GPR103 (extracellular) Antibody (#AGR-033) is a highly specific antibody directed against an epitope of the rat QRFP receptor. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the receptor in living cells. It has been designed to recognize GPCR GPR103 from rat, mouse, and human samples.

Negative Control :

BLP-GR033

Positive Control :

Synonyms :

Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

QRFPR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage