Anti-GPR119 Antibody | Alomone Labs AGR-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR119 Antibody

catalog :

AGR-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat pancreas (lanes 1 and 3) and mouse MS1 cells (lanes 2 and 4): - 1,2. Anti-GPR119 Antibody (#AGR-032), (1:400).3,4. Anti-GPR119 Antibody, preincubated with GPR119 Blocking Peptide (#BLP-GR032).

image 2 :

Peptide CDMLK IASVHSQHIR corresponding to amino acid residues 186-200 of rat GPR119 (AccessionQ7TQN8). 2ndintracellular loop.

product information

CAT :

AGR-032

SKU :

AGR-032-CF_0.2 ml

Product Name :

Anti-GPR119 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7TQN8

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR032

Homology :

Mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CDMLK IASVHSQHIR, corresponding to amino acid residues 186-200 of rat GPR119

Product Page - Scientific background :

Glucose-dependent insulinotropic receptor (GPR119) is a rhodopsin-type member of the G protein-coupled receptor (GPCRs) superfamily. Like all members, it has seven transmembrane spanning domains, an extracellular N-terminus and intracellular C-terminus. Activation of the receptor leads to a rise in intracellular cAMP - suggesting a stimulation of adenylate cyclase, possibly by Gαs coupling. It is expressed mostly in enteroendocrine cells of the gastrointenstinal tract and b-cells of the pancreas. It was stipulated that GPR119 is a close relative to the cannabinoid receptors1,2.Phospholipids and fatty acid amides are endogenous ligands for GPR119. Notably, oleoethanolamide (OEA) seems to be an important agonist. Stimulation of GPR119 by its agonists causes b-cells to release insulin and enteroendocrine cells to release glucagon-like peptide-1 (GLP-1); this attribute makes GPR119 a strong therapeutic candidate for type-2 diabetes and obesity3,4. Furthermore, its ability to release GLP-1 upon stimulation by dietary fat has gained it the title "fat sensor"5 .

Applications may also work in :

Supplier :

Alomone Labs

Target :

Glucose-dependent insulinotropic receptor, GPCR2

Short Description :

A Rabbit Polyclonal Antibody to Glucose-Dependent Insulinotropic Receptor (GPR119)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Glucose-dependent insulinotropic receptor. Anti-GPR119 Antibody (#AGR-032) can be used in western blot analysis. It has been designed to recognize GPR119 from rat, mouse and human samples.

Negative Control :

BLP-GR032

Positive Control :

Synonyms :

Glucose-dependent insulinotropic receptor, GPCR2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPR119

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage