Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody | Alomone Labs AGR-031 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody

catalog :

AGR-031

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Bergmann R, Chollet C, Els Heindl S, Ullrich M, Berndt N, Pietzsch J, et al. Development of a ghrelin receptor inverse agonist for positron emission tomography. Oncotarget. 2021;12:450-474 pubmed publisher

image

image 1 :

Western blot analysis of rat pancreas (lanes 1 and 4), human SH-SY5Y neuroblastoma (lanes 2 and 5) and mouse MS1 endothelial (lanes 3 and 6) cells line lysates: - 1-3. Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody (#AGR-031), (1:200).4-6. Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody, preincubated with Ghrelin Receptor/GHSR (extracellular) Blocking Peptide (#BLP-GR031).

image 2 :

Expression of Ghrelin receptor in rat and mouse hypothalamus - Immunohistochemical staining of rat and mouse hypothalamic paraventricular nucleus (PVN) using Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody (#AGR-031), (1:800). A. Ghrelin receptor 1 staining (red) in mouse appears in cells of the PVN (arrows). B. Ghrelin receptor staining in rat is similar. Nuclear staining using DAPI as the counterstain (blue).

product information

CAT :

AGR-031

SKU :

AGR-031-CF_0.2 ml

Product Name :

Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O08725

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR031

Homology :

Mouse - identical; human - 10/11 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CRATEFAVRSG, corresponding to amino acid residues 197-207 of rat Ghrelin receptor

Product Page - Scientific background :

Ghrelin is a 28 amino acid peptide, acylated on a serine residue which is essential for its biological activity1,2. In rodents, an additional form of the peptide could be purified. The peptide is released from the stomach and exerts its effect on the ghrelin receptor (GHS-R). GHS-R is a member of the G-protein coupled receptor (GPCR) superfamily. Like all members, the receptor has seven transmembrane spanning domains and upon activation it stimulates phospholipase C, thereby increasing IP3 and intracellular Ca2+ levels1,3.GHS-R is expressed in the hypothalamus, pituitary and hippocampus1,3.Some of the actions of ghrelin include the stimulation of growth hormone release, food intake, fat accumulation, regulation of memory and learning, activation of reward-related pathways and neuroprotection to name a few1,3.GHS-R 1b is a truncated form of the full length receptor which is also expressed. Although its exact functions are unknown, it is believed that it may be involved in downregulating signaling from the full length receptor4. In addition, it is overexpressed in lung cancer where it dimerizes with neurotensin receptor4,5.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP

Short Description :

A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR)

Long Description :

Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody (#AGR-031) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize GHSR from human, mouse, and rat samples.

Negative Control :

BLP-GR031

Positive Control :

Synonyms :

Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GHSR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:800

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage