Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody | Alomone Labs AGR-031-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody

catalog :

AGR-031-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Kim D, Yoon Y, Choi D, Kim J, Lim J. 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis. Front Cell Dev Biol. 2021;9:698292 pubmed publisher

image

image 1 :

Cell surface detection of Ghrelin receptor in live intact mouse TK-1 T-cell lymphoma cells: Cell surface detection of Ghrelin receptor in live intact mouse TK-1 T-cell lymphoma cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody (#AGR-031-F), (2.5 µg).

product information

CAT :

AGR-031-F

SKU :

AGR-031-F_50 mcl

Product Name :

Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O08725

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR031

Homology :

Mouse - identical; human - 10 out of 11 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CRATEFAVRSG, corresponding to amino acid residues 197-207 of rat Ghrelin receptor

Product Page - Scientific background :

Ghrelin is a 28 amino acid peptide, acylated on a serine residue which is essential for its biological activity1,2. In rodents, an additional form of the peptide could be purified. The peptide is released from the stomach and exerts its effect on the ghrelin receptor (GHS-R). GHS-R is a member of the G-protein coupled receptor (GPCR) superfamily. Like all members, the receptor has seven transmembrane spanning domains and upon activation it stimulates phospholipase C, thereby increasing IP3 and intracellular Ca2+ levels1,3.GHS-R is expressed in the hypothalamus, pituitary and hippocampus1,3.Some of the actions of ghrelin include the stimulation of growth hormone release, food intake, fat accumulation, regulation of memory and learning, activation of reward-related pathways and neuroprotection to name a few1,3.GHS-R 1b is a truncated form of the full length receptor which is also expressed. Although its exact functions are unknown, it is believed that it may be involved in downregulating signaling from the full length receptor4. In addition, it is overexpressed in lung cancer where it dimerizes with neurotensin receptor4,5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP

Short Description :

A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR) Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody (#AGR-031) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize GHSR from human, mouse, and rat samples. Anti-Ghrelin Receptor (GHSR) (extracellular)-FITC Antibody (#AGR-031-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GHSR

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGR-031), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage