Anti-GnRH Receptor (extracellular) Antibody | Alomone Labs AGR-030 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GnRH Receptor (extracellular) Antibody

catalog :

AGR-030

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat ovary (lanes 1 and 3) and pituitary (lanes 2 and 4) lysates: - 1,2. Anti-GnRH Receptor (extracellular) Antibody (#AGR-030), (1:200).3,4. Anti-GnRH Receptor (extracellular) Antibody, preincubated with GnRH Receptor (extracellular) Blocking Peptide (#BLP-GR030).

image 2 :

Expression of GnRHR in rat ovary - Immunohistochemical staining of rat ovary paraffin-embedded sections using Anti-GnRH Receptor (extracellular) Antibody (#AGR-030). GnRHR labeling appears in the stroma surrounding the follicles. The stroma is a richly cellular connective tissue with scattered smooth muscle fibers. The theca layer is stained as well. Hematoxilin is used as the counterstain.

image 3 :

Expression of GnRHR in rat ovary - Immunohistochemical staining of rat ovary paraffin-embedded sections usingAnti-GnRH Receptor (extracellular) Antibody (#AGR-030). GnRHR labeling appears in the stroma surrounding the follicles. The stroma is a richly cellular connective tissue with scattered smooth muscle fibers. The theca layer is stained as well. Hematoxilin is used as the counterstain.

product information

CAT :

AGR-030

SKU :

AGR-030-CF_0.2 ml

Product Name :

Anti-GnRH Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30969

Applications :

IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR030

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NNASLEQDQNH, corresponding to amino acid residues 3-13 of rat GnRHR

Product Page - Scientific background :

Gonadotropin-releasing hormone (GnRH) or Luteinizing hormone releasing hormone (LHRH) is a decapeptide synthesized and released from neurons in the hypothalamus and stimulates the synthesis and release of gonadotropin hormone (or luteinizing hormone) and follicle stimulating hormone from the pituitary gland1. GnRH is expressed as various variants, and most vertebrates express more than one form of GnRH1-3.GnRH and its variants bind and activate GnRH receptor (GnRHR), a member of the G-protein coupled receptor (GPCR) superfamily. Three forms of the receptor have been identified in vertebrates (types I, II and III). Like all members, GnRHR has seven transmembrane domains and an extracellular N-terminus and an intracellular C-terminal tail. The receptor also has a number of glycosylation sites in its extracellular loops. In addition, the C-terminal tail is unusually short and lacks consensus sites known to undergo post-translational modifications like phosphorylation. In consequence, this affects the receptor's desensitization and internalization4.Expression of GnRHR is detected in the gonadotrope and various tissues like the breast, gonads, prostate and uterus4,5.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Gonadotropin-releasing hormone receptor, GnRHR, Leutinizing hormone releasing hormone receptor, LHRHR, Luliberin receptor

Short Description :

A Rabbit Polyclonal Antibody to GnRH Receptor

Long Description :

Anti-GnRH Receptor (extracellular) Antibody (#AGR-030) is a highly specific antibody directed against an epitope of rat GnRHR. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize GnRHR from mouse and rat samples. It is not suited to recognize GnRHR from human samples.

Negative Control :

BLP-GR030

Positive Control :

Synonyms :

Gonadotropin-releasing hormone receptor, GnRHR, Leutinizing hormone releasing hormone receptor, LHRHR, Luliberin receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GnRHR

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage