Anti-Secretin Receptor (extracellular) Antibody | Alomone Labs AGR-026 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Secretin Receptor (extracellular) Antibody

catalog :

AGR-026

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat pancreas lysate (lanes 3 and 7) and mouse kidney membranes (lanes 4 and 8): - 1-4. Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200). 5-8. Anti-Secretin Receptor (extracellular) Antibody, preincubated with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026).

image 2 :

Expression of Secretin Receptor in mouse cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Secretin Receptor immunoreactivity (green) appears in Bergmann glia profiles (vertical arrows point at Bergmann cell bodies and horizontal arrows at Bergmann glia processes). B. Pre-incubation of the antibody with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026), suppresses staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGR-026

SKU :

AGR-026-CF_0.2 ml

Product Name :

Anti-Secretin Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P23811

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR026

Homology :

Mouse - 14/15 amino acid residues identical; human - 12/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

Peptide (C)NSFNERRHAYLLKLK, corresponding to amino acid residues 129 - 143 of rat Secretin Receptor

Product Page - Scientific background :

Secretin receptor, SCTR, binds Secretin, a hormone that affects water homeostasis throughout the body and specifically in the duodenum of the small intestine.SCTR is ubiquitously expressed throughout the body. It belongs to class B1 of G-protein coupled receptors, along with vasoactive intestinal polypeptide (VIP) receptor. Like all G-protein coupled receptors, it is composed of seven highly conserved transmembrane domains1. The amino terminus of Secretin receptor plays a key role in agonist recognition and responsiveness2.The binding of SCTR to Secretin initiates a signaling cascade that stimulates bicarbonate secretion which in turn leads to neutralization of gastric H+. This process plays an essential role in fat digestion by creating a more neutral (pH 6 to 8) environment. Secretin levels are regulated by H+ and fatty acids levels in the duodenum and hence the secretion regulation is tightly bound to its activity3.In addition, SCTR-Secretin interaction plays a major role in the regulation of the secretory activity in the heart, liver and the central nervous system (CNS)3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

SCTR

Short Description :

A Rabbit Polyclonal Antibody to Secretin Receptor

Long Description :

Anti-Secretin Receptor (extracellular) Antibody (#AGR-026) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SCTR from human, mouse, and rat samples.

Negative Control :

BLP-GR026

Positive Control :

Synonyms :

SCTR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCTR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage