antibody

Anti-GIPR (extracellular) Antibody

AGR-025

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

western blot, immunohistochemistry, immunocytochemistry

AGR-025-CF_0.2 ml

Anti-GIPR (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

P43219

IF IFC IHC LCI WB

Rat Mouse

Rabbit

BLP-GR025

Mouse - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)EGQTTGELYQRWER,corresponding to amino acid residues 25-38 of rat GIPR

Gastric inhibitory polypeptide (GIP) is a 42-amino acid hormone released from the gastrointestinal tract and was first found to inhibit the secretion of hydrochloric acid from stomach parietal cells. GIP also stimulates postprandial insulin secretion from pancreatic β-cells following a pathway activated by the binding of GIP to its specific receptor thus making GIP, along with GLP-1, a major potentiator of glucose induced insulin secretion1.The human GIP receptor is a 466 amino-acid G-coupled protein receptor that is 81.5% and 81.2% identical to the hamster and rat receptors comprising seven potential transmembrane domains. This property is shared with glucagon and secretin receptor families.GIP has an affinity of Kd~0.2 nM to its receptor. GIPR activates several intracellular pathways including MAP kinase, phospholipase A2 but primarily activates adenylyl cyclase causing an increase in cAMP. GIP has been also found to be expressed outside the gut in organs such as the heart, brain and adipose tissue.In patients with Type 2 Diabetes-Mellitus, GIP levels are normal or at most minimally reduced therefore suggesting that there is a resistance of β-cells to GIP caused by a change in receptor expression signaling or mutation. In addition, mice whose GIPR gene expression is disrupted, following three months of high fat feeding, show reduced weight gain and adipose tissue compared to regular mice suggesting the elimination of this receptor may protect against the onset of severe obesity2.

IF IFC IHC LCI WB

Alomone Labs

Gastric inhibitory polypeptide receptor, GIP receptor, Glucose-dependent insulinotropic polypeptide receptor

A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor

Anti-GIPR (extracellular) Antibody (#AGR-025) is a highly specific antibody directed against an epitope of the rat Gastric inhibitory polypeptide receptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially detect the receptor in living cells. It has been designed to recognize GIPR from rat, human, and mouse samples.

BLP-GR025

NA

Gastric inhibitory polypeptide receptor, GIP receptor, Glucose-dependent insulinotropic polypeptide receptor

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

GIPR

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:400

1:200