Anti-Glucagon Receptor (extracellular) Antibody | Alomone Labs AGR-024 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Glucagon Receptor (extracellular) Antibody

catalog :

AGR-024

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Osaka N, Kushima H, Mori Y, Saito T, Hiromura M, Terasaki M, et al. Anti-inflammatory and atheroprotective properties of glucagon. Diab Vasc Dis Res. 2020;17:1479164120965183 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 5), rat liver (lanes 2 and 6), mouse liver (lanes 3 and 7) and human HepG2 liver carcinoma cell line (lanes 4 and 8) lysates: - 1-4. Anti-Glucagon Receptor (extracellular) Antibody (#AGR-024), (1:200).5-8. Anti-Glucagon Receptor (extracellular) Antibody, preincubated with Glucagon Receptor (extracellular) Blocking Peptide (#BLP-GR024).

image 2 :

Expression of Glucagon receptor in mouse hypothalamus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Glucagon Receptor (extracellular) Antibody (#AGR-024), (1:200), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. GCGR staining (green) in mouse hypothalamus is detected in neurons (arrows) from the 3rd ventricle wall outlining the paraventricular nucleus (PVN). Cell nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of rat kidney (lanes 1 and 5) rat liver (lanes 2 and 6) mouse liver (lanes 3 and 7) and human HepG2 liver carcinoma cell line (lanes 4 and 8)lysates: - 1-4.Anti-Glucagon Receptor (extracellular) Antibody (#AGR-024) (1:200).5-8. Anti-Glucagon Receptor (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AGR-024

SKU :

AGR-024-CF_0.2 ml

Product Name :

Anti-Glucagon Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30082

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR024

Homology :

Mouse - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KTRYSQKIGDDLS, corresponding to amino acid residues 200-212 of rat GCGR

Product Page - Scientific background :

The glucagon receptor (GCGR) is one of the 15 members of the secretin-like (class B) family of G-protein-coupled receptors (GPCRs) in humans. Glucagon is a highly conserved 29 amino acid protein processed from proglucagon by PC2 in pancreatic α-cells. The principal actions of glucagon involve regulation of metabolic pathways involved in glucose homeostasis1. Glucagon binding to the GCGR activates adenylyl cyclase through heterotrimeric Gs G proteins, leading to an increase in intracellular cAMP levels and subsequent activation of PKA signaling2. The glucagon receptor is a 485-amino acid protein with a central core consisting of seven membrane-spanning domains, an extracellular N-terminus and a cytoplasmic C-terminal tail2.Glucagon receptors are widely expressed in multiple tissues including the liver, brain, pancreas, heart, kidney, and smooth muscle cell in the gastrointestinal tract and peripheral vasculature1.Abnormal glucagon signaling is associated with hyperglycemia in patients with type 2 diabetes3. Mice with homozygous null mutation of GCGR exhibit chronic hypoglycemia, extreme hyperglucagonemia, and α cell hyperplasia4. GCGR is a potential drug target for type 2 diabetes5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

GCGR, GLR

Short Description :

A Rabbit Polyclonal Antibody to Glucagon Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat glucagon receptor. Anti-Glucagon Receptor (extracellular) Antibody (#AGR-024) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GCGR from rat, mouse and human samples.

Negative Control :

BLP-GR024

Positive Control :

Synonyms :

GCGR, GLR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GCGR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage