Anti-GLP2R (extracellular) Antibody | Alomone Labs AGR-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLP2R (extracellular) Antibody

catalog :

AGR-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Crowley L, Cambuli F, Aparicio L, Shibata M, Robinson B, Xuan S, et al. A single-cell atlas of the mouse and human prostate reveals heterogeneity and conservation of epithelial progenitors. elife. 2020;9: pubmed publisher

image

image 1 :

Western blot analysis of rat colon lysate: - 1. Anti-GLP2R (extracellular) Antibody (#AGR-022), (1:200).2. Anti-GLP2R (extracellular) Antibody, preincubated with GLP2R (extracellular) Blocking Peptide (#BLP-GR022).

image 2 :

Expression of Glucagon-like peptide 2 receptor in rat hypothalamus - Immunohistochemical staining of rat hypothalamus with Anti-GLP2R (extracellular) Antibody (#AGR-022), (1:200). Glucagon-like peptide 2 receptor (green) appears in the lateral hypothalamic region axonal profiles (vertical white arrow) and nerve cell profiles (horizontal white arrows) were labeled. DAPI is used as the counterstain (blue).

image 3 :

Expression of Glucagon-like peptide 2 receptor in rat hypothalamus - Immunohistochemical staining of rat hypothalamus withAnti-GLP2R (extracellular)Antibody (#AGR-022) (1:200). Glucagon-like peptide 2 receptor (green) appears in the lateral hypothalamic region axonal profiles (vertical white arrow) and nerve cell profiles (horizontal white arrows) were labeled. DAPI is used as the counterstain (blue).

product information

CAT :

AGR-022

SKU :

AGR-022-CF_0.2 ml

Product Name :

Anti-GLP2R (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Z0W0

Applications :

IF IHC WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR022

Homology :

Mouse, human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KRPDDESGWMSYLSE, corresponding to amino acid residues 241-255 of rat GLP2R

Product Page - Scientific background :

Glucagon like peptide-2 (GLP-2) is a 33 amino acid peptide secreted following food consumption. It is cleaved by dipeptidyl peptidase IV thereby yielding a very short half life. GLP-2 imposes most of its effect on intestinal epithelial cells where it is also be used as a therapeutic drug for gastrointestinal injuries1-3.The many actions of GLP-2 include cellular proliferation of intestinal epithelial cells, the increase of amino acid and lipid absorption and increase of digestive enzyme activity of the epithelial brush border cells1.The GLP-2 cellular and physiological effects are primarily exerted through the glucagon-like peptide-2 receptor (GLP-2R), a member of the G-protein coupled receptor superfamily. The receptor can couple either Gs or Gi G-proteins1. However, when heterologously expressed, the receptor can stimulate the synthesis of cAMP3.Expression of GLP-2R is strongly detected in the central nervous system and the gastrointestinal tract, although its expression in this area remains controversial as some species express the receptor in different regions than others. It is also slightly detected in the lungs, cervix and vagal afferents1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Glucagon-like peptide 2 receptor, GLP-2 receptor

Short Description :

A Rabbit Polyclonal Antibody to Glucagon-like Peptide 2 Receptor

Long Description :

Anti-GLP2R (extracellular) Antibody (#AGR-022) is a highly specific antibody directed against an epitpe of the rat glucagon-like peptide 2 receptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLP2R from human, rat, and mouse samples.

Negative Control :

BLP-GR022

Positive Control :

Synonyms :

Glucagon-like peptide 2 receptor, GLP-2 receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GLP2R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage