Anti-GLP1R (extracellular) Antibody | Alomone Labs AGR-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GLP1R (extracellular) Antibody

catalog :

AGR-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Ferdaus M, Gratreak B, Miller L, Si J, McCormick J, Yang C, et al. WNK4 limits distal calcium losses following acute furosemide treatment. Physiol Rep. 2019;7:e14195 pubmed publisher
Peiris M, Aktar R, Raynel S, Hao Z, Mumphrey M, Berthoud H, et al. Effects of Obesity and Gastric Bypass Surgery on Nutrient Sensors, Endocrine Cells, and Mucosal Innervation of the Mouse Colon. Nutrients. 2018;10: pubmed publisher
Wang M, Yu X, Lee Y, McCorkle S, Chen S, Li J, et al. Dapagliflozin suppresses glucagon signaling in rodent models of diabetes. Proc Natl Acad Sci U S A. 2017;114:6611-6616 pubmed publisher

image

image 1 :

Western blot analysis of rat pancreas lysate (lanes 1 and 5), mouse preadipocyte 3T3-L1 lysate (lanes 2 and 6), rat pancreatic islet cell line RIN-5F lysate (lanes 3 and 7) and human pancreatic carcinoma PANC-1 lysate (lanes 4 and 8): - 1-4. Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:200).5-8. Anti-GLP1R (extracellular) Antibody, preincubated with GLP1R (extracellular) Blocking Peptide (#BLP-GR021).

image 2 :

Expression of Glucagon-like peptide 1 receptor in rat pancreas - Immunohistochemical staining of rat pancreas paraffin embedded sections using Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:100). Staining (brown color) is present in endocrine cells of the Isles of Langerhans. Hematoxilin is used as the counterstain.

image 3 :

Expression of Glucagon-like peptide 1 receptor in rat pancreatic islet cells - Cell surface detection ofGlucagon-like peptide 1 receptor in live intact rat RIN-5F pancreatic islet cells. A. Cells were stained withAnti-GLP1R (extracellular) Antibody (#AGR-021) (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.

product information

CAT :

AGR-021

SKU :

AGR-021-CF_0.2 ml

Product Name :

Anti-GLP1R (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P32301

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR021

Homology :

Rat, mouse, human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R

Product Page - Scientific background :

Glucagon-like peptide 1 (GLP-1) is a hormone secreted by the intestine following a meal1. This hormone is known to enhance the secretion of insulin, important glucose blood level regulation2,3. In pancreatic β-cells, other than stimulating insulin secretion, GLP-1 action is also important for β-cell mass expansion2.GLP-1 exerts its biological functions through Glucagon-like peptide 1 receptor (GLP-1 receptor), a member of the G-protein coupled receptor superfamily. Like all members, this receptor spans the membrane seven times. GLP-1 receptor couples with Gs, thereby stimulating adenylate cyclase, consequently leading to an increase in cAMP2.The receptor is expressed in various tissues, namely the pancreas, adipose tissue, muscle, heart, gastrointestinal tract and liver. GLP-1 receptor is also found in the central nervous system where it provides a neuroprotective effect4.The glucagon-like peptide 1 system may serve as a therapeutic treatment in diabetes mellitus, in order to maintain β-cell mass expansion2. In addition, the receptor may serve as a therapy target in various neurodegenerative diseases like Alzheimer's and Parkinson's disease4.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Glucagon-like peptide 1 receptor, GLP-1 receptor

Short Description :

A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor

Long Description :

Anti-GLP1R (extracellular) Antibody (#AGR-021) is a highly specific antibody directed against an epitope of the rat glucagon-like peptide 1 receptor. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize GLP1R from human, rat, and mouse samples.

Negative Control :

BLP-GR021

Positive Control :

Synonyms :

Glucagon-like peptide 1 receptor, GLP-1 receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GLP1R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100-1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage