Anti-Glycine Receptor α4 (extracellular) Antibody | Alomone Labs AGR-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Glycine Receptor α4 (extracellular) Antibody

catalog :

AGR-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Lin S, Xie R. Principal Neurons in the Anteroventral Cochlear Nucleus Express Cell-Type Specific Glycine Receptor α Subunits. Neuroscience. 2019;415:77-88 pubmed publisher

image

image 1 :

Western blot analysis of rat eye (lanes 1 and 3) and ARPE-19 (lanes 2 and 4) lysates: - 1,2. Anti-Glycine Receptor α4 (extracellular) Antibody (#AGR-015), (1:500).3,4. Anti-Glycine Receptor α4 (extracellular) Antibody, preincubated with Glycine Receptor α4 (extracellular) Blocking Peptide (#BLP-GR015).

image 2 :

Expression of GlyR?4 in human retinal epithelium (ARPE-19) - Cell surface detection ofGlyR?4 in intact living human ARPE-19 cells. A. Extracellular staining of cells usingAnti-Glycine Receptor ?4 (extracellular) Antibody (#AGR-015) (1:50) followed by goat anti-rabbit-AlexaFluor-594secondary antibody. B. Merge image of A and live view of the cells.

image 3 :

Western blot analysis of rat eye (lanes 1 and 3) and ARPE-19 (lanes 2 and 4) lysates: - 12.Anti-Glycine Receptor ?4 (extracellular)Antibody (#AGR-015) (1:500).34. Anti-Glycine Receptor ?4 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AGR-015

SKU :

AGR-015-CF_0.2 ml

Product Name :

Anti-Glycine Receptor α4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61603

Applications :

IC IF LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR015

Homology :

Rat - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KEDVKSGLKGSQPMS, corresponding to amino acid residues 28-42 of mouse GlyRα4

Product Page - Scientific background :

The amino acid glycine mediates neuronal inhibition by activating glycine receptors (GlyRs), which are ligand-gated chloride channels of the nicotinic acetylcholine receptor superfamily. GlyRs are known to mediate postsynaptic inhibition in spinal cord, brain stem and some higher brain regions1. GlyR is a member of the pentameric Cys-loop ion channel receptor family. Functional Cys-loop receptors comprise homomeric or heteromeric pentameric oligomers with each of the five subunits arranged symmetrically in a ring around a central ion-conducting pore. Several developmentally and regionally regulated GlyR isoforms exist, which result from a differential expression of the GlyR α (α1- α4) and β subunit genes. Each GlyR subunit comprises a large extracellular amino-terminal domain that harbors the ligand-binding sites and the eponymous Cys-loop. This connects to a bundle of four α-helical transmembrane domains (labelled M1-M4) with a large intracellular domain between M3 and M4 and a short extracellular C-terminal tail2. The human α4 subunit is considered a pseudogene on the grounds that it incorporates a premature stop codon upstream of the final TM4 domain3.The α4 subunit has been found in the adult mouse retina where it clusters together with synaptic markers such as bassoon or gephyrin. Furthermore, GlyR α4 transcripts have been detected in the white matter tract of the developing spinal cord from mice. The avian α4 mRNA was also found in dorsal root ganglia and genital ridge 4.Although the function of GlyR α4 at its sites of expression is still unknown, homomeric α4 receptors are able to form fully functional glycine-gated Cl− channels in vitro that can be antagonized with strychnine5.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

Glycine receptor subunit alpha-4, GLRA4

Short Description :

A Rabbit Polyclonal Antibody to Glycine Receptor α4

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the mouse glycine receptor α4. Anti-Glycine Receptor α4 (extracellular) Antibody (#AGR-015) can be used in western blot and live cell imagine applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting GlyRα4 in living cells. It has been designed to recognize GlyRα4 from human, rat and mouse samples.

Negative Control :

BLP-GR015

Positive Control :

Synonyms :

Glycine receptor subunit alpha-4, GLRA4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GLRA4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage