Anti-Glycine Receptor β Antibody | Alomone Labs AGR-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Glycine Receptor β Antibody

catalog :

AGR-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Araya A, Gallegos S, Viveros R, San Martin L, Muñoz B, Harvey R, et al. Presence of ethanol-sensitive and ethanol-insensitive glycine receptors in the ventral tegmental area and prefrontal cortex in mice. Br J Pharmacol. 2021;178:4691-4707 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-Glycine Receptor β Antibody (#AGR-014), (1:200).3,4. Anti-Glycine Receptor β Antibody, preincubated with Glycine Receptor β Blocking Peptide (#BLP-GR014).

image 2 :

Peptide (C)SHITRRPNGKTWSTKR corresponding to amino acid residues 656-671 of rat NLGN3 (Accession Q62889). Extracellular N-terminus.

product information

CAT :

AGR-014

SKU :

AGR-014-CF_0.2 ml

Product Name :

Anti-Glycine Receptor β Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P20781

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR014

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

HISTLQVGETRC, corresponding to amino acid residues 394-405 of rat GlyRβ

Product Page - Scientific background :

The amino acid glycine mediates neuronal inhibition by activating glycine receptors (GlyRs), which are ligand-gated chloride channels of the nicotinic acetylcholine receptor superfamily. GlyRs are known to mediate postsynaptic inhibition in spinal cord, brain stem and some higher brain regions1. GlyRs are members of the pentameric Cys-loop family of ionotropic neurotransmitter receptors.GlyRs are composed of homologous α (α1-α4) and β subunits. Upon synthesis at the endoplasmic reticulum (ER), all GlyRα subunits can assemble into functional homopentamers that are inserted into the plasma membrane. In contrast, the GlyRβ subunit requires stoichiometric coassembly with GlyRα subunits for ER exit and cell surface expression. Each subunit consists of an extended N-terminal extracellular domain, four transmembrane domains, and a large intracellular loop between transmembrane domains 3 and 4. The β subunit is responsible for synaptic localization of the GlyRs by interacting with the postsynaptic scaffolding protein gephyrin. GlyRβ subunit is reported to interact with the proteins Vacuolar Protein Sorting 35 (Vps35) and Neurobeachin (Nbea)2.GlyRβ mRNA is abundantly found throughout the late embryonic and postnatal brain3.Mutations in GLRB, the gene encoding GlyRβ, have been discovered in patients with hyperekplexia4, in the mouse mutant spastic5 and the zebrafish bandoneon mutant6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Glycine receptor subunit beta, Glycine receptor 58 kDa subunit, GLRB, GlyRβ

Short Description :

A Rabbit Polyclonal Antibody to Glycine Receptor β

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat glycine receptor β. Anti-Glycine Receptor β Antibody (#AGR-014) can be used in western blot analysis. It has been designed to recognize GlyRβ from human, rat and mouse samples.

Negative Control :

BLP-GR014

Positive Control :

Synonyms :

Glycine receptor subunit beta, Glycine receptor 58 kDa subunit, GLRB, GlyRβ

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GLRB

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage