Anti-GALR2 Antibody | Alomone Labs AGR-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GALR2 Antibody

catalog :

AGR-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Hu J, Gao Y, Huang Q, Wang Y, Mo X, Wang P, et al. Flotillin-1 Interacts With and Sustains the Surface Levels of TRPV2 Channel. Front Cell Dev Biol. 2021;9:634160 pubmed publisher
Flores Burgess A, Millón C, Gago B, García Durán L, Cantero García N, Coveñas R, et al. Galanin (1-15)-fluoxetine interaction in the novel object recognition test. Involvement of 5-HT1A receptors in the prefrontal cortex of the rats. Neuropharmacology. 2019;155:104-112 pubmed publisher
Narvaez M, Borroto Escuela D, Santín L, Millón C, Gago B, Flores Burgess A, et al. A Novel Integrative Mechanism in Anxiolytic Behavior Induced by Galanin 2/Neuropeptide Y Y1 Receptor Interactions on Medial Paracapsular Intercalated Amygdala in Rats. Front Cell Neurosci. 2018;12:119 pubmed publisher
Weyne E, Hannan J, Gevaert T, Soebadi M, Matsui H, Castiglione F, et al. Galanin Administration Partially Restores Erectile Function After Cavernous Nerve Injury and Mediates Endogenous Nitrergic Nerve Outgrowth InÂ Vitro. J Sex Med. 2018;15:480-491 pubmed publisher

image

image 1 :

Western blot analysis of rat hippocampus (lanes 1 and 4), rat DRG (lanes 2 and 5), rat brain (lanes 3 and 6), mouse brain (lanes 7 and 9) and human prostate carcinoma LNCaP cell lysates (lanes 8 and 10): - 1,2,3,7,8. Anti-GALR2 Antibody (#AGR-012), (1:200).4,5,6,9,10. Anti-GALR2 Antibody, preincubated with GALR2 Blocking Peptide (#BLP-GR012).

image 2 :

Expression of Galanin receptor 2 in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-GALR2 Antibody (#AGR-012), (1:100). GALR2 is expressed in the DRG neurons. Hoechst 33342 is used as the counterstain.

product information

CAT :

AGR-012

SKU :

AGR-012-CF_0.2 ml

Product Name :

Anti-GALR2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88854

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR012

Homology :

Rat - identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GSGSQRAKRKVTR, corresponding to amino acid residues 223-235 of mouse Galanin receptor type 2

Product Page - Scientific background :

Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.1-4GALR2 is widely expressed in the brain (with highest levels of mRNA found in the hypothalamus, hippocampus, amygdala and pyriform cortex) and in peripheral tissues such as the small intestine, heart, kidney, and liver. High levels of GALR2 expression were also found in rat dorsal root ganglia.1GALR2 activation increases inositol phosphate hydrolysis, mediates the release of Ca2+ into the cytoplasm from intracellular stores, and opens Ca2+-dependent chloride channels in a PTX-resistant manner, indicating that GALR2 couples to Gq/11 proteins.5

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Galanin receptor type 2, GALNR2, GAL2R

Short Description :

A Rabbit Polyclonal Antibody to Galanin Receptor Type 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse Galanin receptor 2. Anti-GALR2 Antibody (#AGR-012) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GALR2 from mouse, rat and human samples.

Negative Control :

BLP-GR012

Positive Control :

Synonyms :

Galanin receptor type 2, GALNR2, GAL2R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GALR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage