Anti-GALR1 Antibody | Alomone Labs AGR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GALR1 Antibody

catalog :

AGR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Hu J, Gao Y, Huang Q, Wang Y, Mo X, Wang P, et al. Flotillin-1 Interacts With and Sustains the Surface Levels of TRPV2 Channel. Front Cell Dev Biol. 2021;9:634160 pubmed publisher
Weyne E, Hannan J, Gevaert T, Soebadi M, Matsui H, Castiglione F, et al. Galanin Administration Partially Restores Erectile Function After Cavernous Nerve Injury and Mediates Endogenous Nitrergic Nerve Outgrowth InÂ Vitro. J Sex Med. 2018;15:480-491 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-GALR1 Antibody (#AGR-011), (1:200).2. Anti-GALR1 Antibody, preincubated with GALR1 Blocking Peptide (#BLP-GR011).

image 2 :

Expression of Galanin receptor 1 in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen section using Anti-GALR1 Antibody (#AGR-011), (1:200), (red). Galanin receptor 1 is expressed in DRG neuron cells. Hoechst 33342 is used as the counterstain (blue).

image 3 :

Expression of Galanin receptor 1 in ratDRG - Immunocytochemical staining of GALR1 in a primary culture of rat dorsal root ganglion (DRG) neurons. A D. Paraformaldehyde-fixed and permeabilized DRG primary culture stained with Anti-GALR1 Antibody(#AGR-011) (1:100) followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). B E. Nuclear fluorescence staining of cells using Hoechst 33342(blue).C. Merged images of panels A and B.F. Merged images ofpanels D and E.

product information

CAT :

AGR-011

SKU :

AGR-011-CF_0.2 ml

Product Name :

Anti-GALR1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P56479

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR011

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

RSKPGKPRSTTN(C), corresponding to amino acid residues 60-71 of mouse Galanin receptor type 1

Product Page - Scientific background :

Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.1-4Based on mRNA, the GALR1 is predominantly distributed in the central nervous system, spinal cord and dorsal root ganglia while GALR2 is abundant in both central and peripheral nervous systems. GALR3 mRNA is distributed in peripheral tissues such as the heart, spleen, and testis. However, its expression in the brain is still controversial.4,5

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Galanin receptor type 1, GALNR1, GAL1R

Short Description :

A Rabbit Polyclonal Antibody to Galanin Receptor Type 1

Long Description :

Anti-GALR1 Antibody (#AGR-011) is a highly specific antibody directed against an epitope of the mouse Galanin receptor 1. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize GALR1 from mouse, rat, and human samples.

Negative Control :

BLP-GR011

Positive Control :

Synonyms :

Galanin receptor type 1, GALNR1, GAL1R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GALR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage