Anti-Glycine Receptor α3 Antibody | Alomone Labs AGR-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Glycine Receptor α3 Antibody

catalog :

AGR-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Lin S, Xie R. Principal Neurons in the Anteroventral Cochlear Nucleus Express Cell-Type Specific Glycine Receptor α Subunits. Neuroscience. 2019;415:77-88 pubmed publisher
Bae J, Mah W, Rah J, Park S, Bae Y. Expression of glycine receptor alpha 3 in the rat trigeminal neurons and central boutons in the brainstem. Brain Struct Funct. 2016;221:4601-4613 pubmed

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:200).3-4. Anti-Glycine Receptor α3 Antibody, preincubated with Glycine Receptor α3 Blocking Peptide (BLP-GR003).

image 2 :

Western blot analysisof rat brain membranes: - 1.Anti-Glycine Receptor ?3 Antibody (#AGR-003) (1:200).2. Anti-Glycine Receptor ?3 Antibody preincubated with the control peptide antigen.

image 3 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 12. Anti-NCAM2 (extracellular) Antibody (extracellular)Antibody (#ANR-042) (1:200).34. Anti-NCAM2 (extracellular) Antibody (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AGR-003

SKU :

AGR-003-CF_0.2 ml

Product Name :

Anti-Glycine Receptor α3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P24524

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR003

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KNKTEAFALEKFYR, corresponding to amino acid residues 355-368 of rat GlyRα3

Product Page - Scientific background :

Glycine is a major neurotransmitter in the mammalian central nervous system.1 The glycine receptor is a ligand gated Cl- channel, mediating synaptic inhibition in the spinal cord, retina and regions of the brain.2The glycine receptor is a pentamer consisting of α and β subunits. To date there are four known α-subunit isoforms (α1-α4) encoded by different genes.2-4 In humans, two alternative splice variants of the glycine receptor α3 have been described: α3L and α3K, where the α3L splice variant bears an additional segment of 15 amino acids within the long cytoplasmic TM3-4 loop. These variants differ in their desensitization kinetics.3

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Glycine receptor subunit alpha-3, GLRA3, GlyRα3

Short Description :

A Rabbit Polyclonal Antibody to Glycine Receptor α3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat glycine receptor α3. Anti-Glycine Receptor α3 Antibody (#AGR-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GlyRα3 from rat, mouse and human samples.

Negative Control :

BLP-GR003

Positive Control :

Synonyms :

Glycine receptor subunit alpha-3, GLRA3, GlyRα3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GLRA3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage