Anti-Glycine Receptor α1 Antibody | Alomone Labs AGR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Glycine Receptor α1 Antibody

catalog :

AGR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Wang H, Cheng K, Chen P, Tseng K, Kwan A, Chang L. Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models. BMC Neurosci. 2018;19:72 pubmed publisher

image

image 1 :

Western blot analysis of rat DRG (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates: - 1-3. Anti-Glycine Receptor α1 Antibody (#AGR-001), (1:400).4-6. Anti-Glycine Receptor α1 Antibody, preincubated with Glycine Receptor α1 Blocking Peptide (#BLP-GR001).

image 2 :

Expression of GlyR α1 in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-Glycine receptor α1 Antibody (#AGR-001), (1:100). A. GlyRα1 staining (red) is revealed in the molecular layer (MOL), Bergmann glia (horizontal arrow) and in Purkinje cells (a vertical arrow). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of A and B.

image 3 :

Expression of GlyR ?1 in rat cerebellum - Immunohistochemical staining of rat cerebellum usingAnti-Glycine receptor?1Antibody (#AGR-001) (1:100). A. GlyR?1 staining (red) is revealed in the molecular layer (MOL) Bergmann glia (horizontal arrow) and in Purkinje cells (a vertical arrow). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of A and B.

product information

CAT :

AGR-001

SKU :

AGR-001-CF_0.2 ml

Product Name :

Anti-Glycine Receptor α1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P07727

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GR001

Homology :

Mouse - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RHHKSPMLNLFQD, corresponding to amino acid residues 350-362 of rat GlyRα1

Product Page - Scientific background :

Glycine receptors (GlyRs) mediate ionotropic inhibitory neurotransmission in the CNS where they play an essential role in inhibition of motor neurons in the spinal cord and brainstem1. The glycine receptor is composed of three ligand-binding subunits of 48 kDa (α) and two homologous polypeptides of 58 kDa (β) that span the postsynaptic membrane in a pentameric arrangement to form a Cl--selective channel2. So far, at least three different α-subunit variants of the GlyR have been identified: α1, α2 and α33. Homo-oligomeric α2 receptors have a low strychnine-binding affinity and are considered to represent a neonatal form of GlyR, which is replaced during development by receptors containing the α1 subunit. Adult GlyRs are predominantly composed of α1 and β subunits, the α3 subunit being expressed at low levels in only a few brain regions4.GlyRα1 subunits are widely expressed and contribute to many processes in the CNS, including inflammatory pain perception, modulation of auditory and visual pathways, and neurotransmission in the cerebellar cortex5.Mutations in glycine receptor α1 subunits cause human hereditary hyperekplexia, a disorder characterized by an exaggerated startle response, which is a consequence of the role of GlyRs in motor reflex circuits of the spinal cord6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Glycine receptor subunit alpha-1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit, GLRA1, Glyr

Short Description :

A Rabbit Polyclonal Antibody to Glycine Receptor α1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat glycine receptor α1. Anti-Glycine Receptor α1 Antibody (#AGR-001) can be used in western blot and immunohistochemical staining. It has been designed to recognize GlyRα1 from rat, mouse and human samples.

Negative Control :

BLP-GR001

Positive Control :

Synonyms :

Glycine receptor subunit alpha-1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit, GLRA1, Glyr

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GLRA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage