Anti-GSG1L Antibody | Alomone Labs AGC-048 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GSG1L Antibody

catalog :

AGC-048

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1, 2. Anti-GSG1L Antibody (#AGC-048), (1:200). 3, 4. Anti-GSG1L Antibody, preincubated with GSG1L Blocking Peptide (#BLP-GC048).

image 2 :

Expression of GSG1L in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GSG1L Antibody (#AGC-048), (1:200), followed by goat anti-rabbit-AlexaFluor-488. GSG1L immunoreactivity (green) appears in the outer molecular layer (OML) and in the granule layer (GL) of the hippocampal dentate gyrus. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGC-048

SKU :

AGC-048-CF_0.2 ml

Product Name :

Anti-GSG1L Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

D3ZK93

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC048

Homology :

Mouse - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CRHERYPTRHQPH, corresponding to amino acid residues 283 - 295 of rat GSG1L

Product Page - Scientific background :

Germline-specific gene 1-like (GSG1L) is a tetra-spanning membrane protein which belongs to the Claudin protein superfamily. It acts as an auxiliary subunit that modulates the assembly, trafficking and gating of AMPA receptors (AMPAR)1.GSG1L has four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm, the juxtamembrane region in its C-tail extracellular Loop1 domain is critical for its suppression of AMPAR-mediated synaptic transmission2.AMPA receptors are ligand gated ion channels critical for synaptic transmission and plasticity. Their dysfunction is involved in a variety of psychiatric and neurological diseases ranging from major depressive disorder to amyotrophic lateral sclerosis. A newly explored strategy is to manipulate the activity of AMPA receptors is through compounds that affect specific AMPAR-auxiliary subunit complexes. Overexpression of GSG1L strongly suppresses AMPAR, and GSG1L knockout enhances AMPAR-mediated synaptic transmission which makes it a leading therapeutic candidate for manipulating AMPAR activity3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Germ cell-specific gene 1-like protein, GSG1-like protein

Short Description :

A Rabbit Polyclonal Antibody to GSG1L

Long Description :

Anti-GSG1L Antibody (#AGC-048) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GSG1L from human, rat, and mouse samples.

Negative Control :

BLP-GC048

Positive Control :

Synonyms :

Germ cell-specific gene 1-like protein, GSG1-like protein

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GSG1L

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage