Anti-CNIH2/3 (extracellular) Antibody | Alomone Labs AGC-047 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CNIH2/3 (extracellular) Antibody

catalog :

AGC-047

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-CNIH2/3 (extracellular) Antibody (#AGC-047), (1:200).3,4. Anti-CNIH2/3 (extracellular) Antibody, preincubated with CNIH2/3 (extracellular) Blocking Peptide (#BLP-GC047).

product information

CAT :

AGC-047

SKU :

AGC-047-CF_0.2 ml

Product Name :

Anti-CNIH2/3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q5BJU5 and D0Q0Y7

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC047

Homology :

CNIH2 = Mouse, human identicalCNIH3 = Rat, mouse, human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

RARERLKNIERIC, corresponding to amino acid residues 49-61 of rat CNIH2

Product Page - Scientific background :

Cornichon homologs (CNIHs) are a family of AMPA-type glutamate receptor (AMPAR) auxiliary subunits that modulate AMPAR ion channel function and trafficking. Cornichon 2 and 3 (CNIH2/3) are responsible for controlling the export of AMPARs from the endoplasmic reticulum and associate with synaptic AMPARs to modulate channel kinetics1,2.CNIHs are small proteins with three transmembrane domains that directly binds to AMPARs. Overexpression CNIHs and AMPA receptors causes their localization to the Golgi apparatus3.CNIH and TARP both compete for the binding to AMPAR subunits GluA2, GluA3, and GluA4 subunits suggesting both proteins bind to the same site. CNIH2/3 binding to AMPARs is dependent on AMPAR subunit composition and TARPs3,4.CNIH2/3 knock out mice shows alterations in synaptic content of GluA1-containing AMPARs, resulting in a reduction in AMPAR-mediated transmission and long-term potentiation2.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Cornichon homolog 2, Cornichon homolog 3, Cornichon family AMPA receptor auxiliary protein 2, Cornichon family AMPA receptor auxiliary protein 3

Short Description :

A Rabbit Polyclonal Antibody to CNIH2 and CNIH3

Long Description :

Anti-CNIH2/3 (extracellular) Antibody (#AGC-047) is a highly specific antibody directed against a conserved sequence of the rat CNIH2 and CNIH3 proteins. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and thus is ideal for detecting the proteins in living cells. It has been designed to recognize CNIH2 and CNIH3 from human, rat, and mouse samples.

Negative Control :

BLP-GC047

Positive Control :

Synonyms :

Cornichon homolog 2, Cornichon homolog 3, Cornichon family AMPA receptor auxiliary protein 2, Cornichon family AMPA receptor auxiliary protein 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CNIH2, CNIH3

AB Specifictiy :

This antibody will recognize both CNIH2 and CNIH3, but not the related proteins CNIH1 and CNIH4.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage