Anti-NETO1 (extracellular) Antibody | Alomone Labs AGC-045 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NETO1 (extracellular) Antibody

catalog :

AGC-045

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2 Anti-NETO1 (extracellular) Antibody (#AGC-045), (1:200).3,4. Anti-NETO1 (extracellular) Antibody, preincubated with NETO1 (extracellular) Blocking Peptide (#BLP-GC045).

image 2 :

Expression of NETO1 in rat and mouse cortex. - Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-NETO1 (extracellular) Antibody (#AGC-045), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. NETO1 staining in rat (A) and mouse (B) sections (green), is detected in cells in the deep pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGC-045

SKU :

AGC-045-CF_0.2 ml

Product Name :

Anti-NETO1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8R4I7

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC045

Homology :

Rat - identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KKGTEKQITPETQK, corresponding to amino acid residues 24-37 of mouse NETO1

Product Page - Scientific background :

NETO proteins are auxiliary subunits that play crucial roles in modulating the biophysical properties of Kainate receptors (KARs) and N-methyl-D-aspartate receptors (NMDARs). NETO proteins modulate these receptors both in vivo and in vitro. NETO1 is also responsible for the slow kinetics of postsynaptic KAR-mediated currents and regulate synaptic recruitment of Kainate receptors1.NETO1 is a single transmembrane domain protein containing two extracellular CUB domains of about 110 amino acids, a low-density lipoprotein receptor domain, and a cytoplasmic tail of almost 167 amino acids that includes a C-terminal TRV class I PDZ binding motif2.NETO1 and NETO2, are highly homologous, but have a different expression patterns and distinct functional effects. NETO1 subunit is expressed in high levels in the developing hippocampus and regulates axonal and presynaptic Kainate receptors at the CA3-CA1 region of the hippocampus. NETO1 deficiency resulted in impairs synaptogenesis and disrupts synchronization of the CA3-CA1 neuronal populations1,3. NETO1 is enriched in the post-synaptic density where it co-localizes with post-synaptic density-95 (PSD-95) and GluN12.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, BCTL1, BTCL1

Short Description :

A Rabbit Polyclonal Antibody to NETO1

Long Description :

Anti-NETO1 (extracellular) Antibody (#AGC-045) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize NETO1 from rat, mouse, and human samples.

Negative Control :

BLP-GC045

Positive Control :

Synonyms :

Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, BCTL1, BTCL1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NETO1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage