Anti-GRIK5 (GluK5) (extracellular) Antibody | Alomone Labs AGC-042 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GRIK5 (GluK5) (extracellular) Antibody

catalog :

AGC-042

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Bhandage A, Jin Z, Hellgren C, Korol S, Nowak K, Williamsson L, et al. AMPA, NMDA and kainate glutamate receptor subunits are expressed in human peripheral blood mononuclear cells (PBMCs) where the expression of GluK4 is altered by pregnancy and GluN2D by depression in pregnant women. J Neuroimmunol. 2017;305:51-58 pubmed publisher

image

image 1 :

Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 1,2. Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042), (1:400).3,4. Anti-GRIK5 (GluK5) (extracellular) Antibody, preincubated with GRIK5/GluK5 (extracellular) Blocking Peptide (#BLP-GC042).

image 2 :

Expression of kainate receptor GluK5 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042), (1:300), followed by goat-anti-rabbit-Alexa-488. GluK5 staining (green) appears in neurons (horizontal arrows) of the pyramidal layer (Pyr) and in apical dendrites (vertical arrows) of mouse hippocampus. Nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 12.Anti-GRIK5 (GluK5) (extracellular) Antibody(#AGC-042) (1:400).34. Anti-GRIK5 (GluK5) (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AGC-042

SKU :

AGC-042-CF_0.2 ml

Product Name :

Anti-GRIK5 (GluK5) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63273

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC042

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RRPNFQALSGNER,corresponding to amino acid residues 430-442 of rat kainate receptor GluK5

Product Page - Scientific background :

There are three glutamate receptor superfamilies defined by the specific agonist binding to them - NMDA, AMPA and kainate.Several types of kainate receptors have been cloned and classified. Two of these have a high affinity to kainate (GLUK1 and GLUK2), while GLUK5, as well as other members of the family, have a lower affinity1. GLUK5, GLUK6 and GLUK7 have a 75-80% identical amino acid sequence making them a separate subfamily from the other kainate receptors.The GLUK5 receptor, similar to its subfamily members, is a ligand-gated ion channel. It has an extracellular N-terminus comprised of eight glycosylation consensus sites, four transmembrane domains arranged in a three-plus-one pattern with a large intracellular domain between units three and four and a C-terminus2. GLUK5 is expressed in DRG neuron cell bodies where currents in these cells are preferentially induced by kainate and not AMPA3 and also in trigeminal neurons where kainate receptor antagonists are effective in a dural plasma protein extravasation model of migraine in rats4.Little is known about the role of GLUK5 in pathologies. A study examining the role of the receptor in brain pathology using GLUK5 antagonists on hippocampal cells slices, found that blocking of the kainate receptor prevents the development of epileptic activity evoked by muscarinic antagonist. The administration of GLUK5 antagonists to conscious mice in the same study showed that the antagonist prevents limbic seizures induced by hippocampal pilocarpine perfusion without evident side effects. These findings suggest that GLUK5 antagonists may be effective in the treatment of epilepsy and further research in this field is required5.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Glutamate receptor ionotropic kainate 5, Glutamate receptor KA-2, KA2

Short Description :

A Rabbit Polyclonal Antibody to Kainate Receptor GluK5

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat kainate receptor GluK5. Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GluK5 from rat, human, and mouse samples.

Negative Control :

BLP-GC042

Positive Control :

Synonyms :

Glutamate receptor ionotropic kainate 5, Glutamate receptor KA-2, KA2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIK5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage