Anti-GRIK4 (KA1) (extracellular) Antibody | Alomone Labs AGC-041 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GRIK4 (KA1) (extracellular) Antibody

catalog :

AGC-041

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Mukherjee A, Lam N, Wimmer R, Halassa M. Thalamic circuits for independent control of prefrontal signal and noise. Nature. 2021;600:100-104 pubmed publisher
Bhandage A, Jin Z, Hellgren C, Korol S, Nowak K, Williamsson L, et al. AMPA, NMDA and kainate glutamate receptor subunits are expressed in human peripheral blood mononuclear cells (PBMCs) where the expression of GluK4 is altered by pregnancy and GluN2D by depression in pregnant women. J Neuroimmunol. 2017;305:51-58 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-GRIK4 (KA1) (extracellular) Antibody (#AGC-041), (1:400).3,4. Anti-GRIK4 (KA1) (extracellular) Antibody, preincubated with GRIK4/KA1 (extracellular) Blocking Peptide (#BLP-GC041).

image 2 :

Expression of kainate receptor GluK4 in rat cerebellar deep nuclei - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GRIK4 (KA1) (extracellular) Antibody (#AGC-041), (1:100). A. GluK4 staining (red) appears in neurons (arrows) of deep cerebellar nuclei. B. Cell nuclei in the same section are visualized with DAPI (blue). C. Merge of the two images.

image 3 :

Peptide (C)KNRINRAPERLGK corresponding to amino acid residues 49-61 of rat kainate receptor GluK4 (AccessionQ01812). Extracellular N-terminus.

product information

CAT :

AGC-041

SKU :

AGC-041-CF_0.2 ml

Product Name :

Anti-GRIK4 (KA1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q01812

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC041

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KNRINRAPERLGK, corresponding to amino acid residues 49-61 of rat kainate receptor GluK4

Product Page - Scientific background :

Glutamate is the principal excitatory neurotransmitter in the central nervous system (CNS). Glutamate is involved in cognitive functions like learning and memory. Imbalances in glutamatergic transmission have profound physiological and behavioral consequences1.Ionotropic glutamate receptors are classified functionally and by molecular homology into three receptor classes: N-methyl-D-aspartate (NMDA), amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), and kainate (KA)2. KA receptors assemble as tetramers from five subunit types, GluK1, GluK2 GluK3, GluK4 and GluK5. The GluK1-GluK3 subunits have low glutamate affinity and are capable of forming functional homomeric channels. GluK4 and GluK5 are high-affinity kainate receptor subunits that bind glutamate but require coassembly with one or more GluK1-GluK3 subunits to form functional channels. The heteromultimeric assembly of kainate receptors like many other ion channels leads to the formation of receptors with unique pharmacological and functional properties3. Unlike the other KA receptor subunits, which are expressed throughout the CNS, GluK4 expression is limited to only a few regions of the brain, and expression is highest in the CA3 region of the hippocampus and dentate gyrus4.The GluK4 receptor subunit gene, GRIK4, is located near the tip of the long arm of chromosome 11. Genetic variants in the GRIK4 gene have been demonstrated to associate with several psychiatric disorders. GRIK4 has been identified as a susceptibility gene in schizophrenia and bipolar disorder5. GluK4 may also play a role in excitotoxic neurodegeneration6.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Glutamate receptor ionotropic kainate 4, GluK4, Glutamate receptor KA-1

Short Description :

A Rabbit Polyclonal Antibody to Kainate Receptor GluK4

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat kainate receptor GluK4. Anti-GRIK4 (KA1) (extracellular) Antibody (#AGC-041) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize GluK4 from rat, mouse, and human samples.

Negative Control :

BLP-GC041

Positive Control :

Synonyms :

Glutamate receptor ionotropic kainate 4, GluK4, Glutamate receptor KA-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIK4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage