Anti-GRIK3 (GluK3) (extracellular) Antibody | Alomone Labs AGC-040 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GRIK3 (GluK3) (extracellular) Antibody

catalog :

AGC-040

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Junyent S, Reeves J, Gentleman E, Habib S. Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells. J Cell Biol. 2021;220: pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040), (1:200).3,4. Anti-GRIK3 (GluK3) (extracellular) Antibody, preincubated with GRIK3/GluK3 (extracellular) Blocking Peptide (#BLP-GC040).

image 2 :

Expression of GluR7 in mouse hippocampus and rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating frozen brain sections using Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040), (1:400). A. GluR7 staining (red) in mouse hippocampus reveals expression in the pyramidal (P) layer (arrow). B. GluR7 staining (red) in rat cerebellum reveals expression in the Purkinje (P) layer (horizontal arrow) and in the molecular layer (MOL) interneurons (arrow).

product information

CAT :

AGC-040

SKU :

AGC-040-CF_0.2 ml

Product Name :

Anti-GRIK3 (GluK3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P42264

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC040

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DGPNAQVMNAEEH, corresponding to amino acid residues 46-58 of rat kainate receptor GluK3

Product Page - Scientific background :

Ionotropic glutamate receptors (iGluRs) mediate the majority of fast excitatory synaptic neurotransmission in the central nervous system. The selective assembly of iGluRs into AMPA, kainate, and N-methyl-D-aspartic acid (NMDA) receptor subtypes is regulated by their extracellular amino-terminal domains (ATDs)1.Kainate receptors (KARs) are tetramers classified into low-affinity receptor families (GluK1-GluK3) and high-affinity receptor families (GluK4-GluK5) based on their affinity for the neurotoxin kainic acid. KARs share a similar architecture with other ionotropic glutamate receptors; the subunits have a large extracellular domain composed of an amino-terminal domain (ATD) and a ligand binding domain (LBD) and an intracellular carboxy-terminal region2.KARs are expressed in neurons and glial cells throughout the CNS. GluK3 is poorly expressed, appearing in layer IV of the neocortex and dentate gyrus in the hippocampus3.KARs serve a crucial role as modulators of synaptic transmission and plasticity and their dysfunction has been linked to several disease states such as epilepsy, chronic pain and neurodegenerative diseases4. Alteration in the extracellular domain of GluK3, is in linkage disequilibrium with recurrent major depressive disorder patients5 and subjects with schizophrenia6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Glutamate receptor ionotropic kainate 3, Glutamate receptor 7, GluR7

Short Description :

A Rabbit Polyclonal Antibody to Kainate Receptor GluK3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat kainate receptor GluK3. Anti-GRIK3 (GluK3) (extracellular) Antibody (#AGC-040) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus highly suited to recognize the receptor in living cells. It has been designed to recognize GluR7 from human, rat and mouse samples.

Negative Control :

BLP-GC040

Positive Control :

Synonyms :

Glutamate receptor ionotropic kainate 3, Glutamate receptor 7, GluR7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIK3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage