Anti-GRID2 (extracellular) Antibody | Alomone Labs AGC-039 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GRID2 (extracellular) Antibody

catalog :

AGC-039

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 1a	. Absence of GluD2 Antibodies in Patients With Opsoclonus-Myoclonus Syndrome. Neurology. 2021;96:e1082-e1087 pubmed publisher
	Pernice H, Schieweck R, Jafari M, Straub T, Bilban M, Kiebler M, et al. Altered Glutamate Receptor Ionotropic Delta Subunit 2 Expression in Stau2-Deficient Cerebellar Purkinje Cells in the Adult Brain. Int J Mol Sci. 2019;20: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human U87-MG glioblastoma cell lysate (lines 3 and 6): - 1-3. Anti-GRID2 (extracellular) Antibody (#AGC-039), (1:600).4-6. Anti-GRID2 (extracellular) Antibody, preincubated with GRID2 (extracellular) Blocking Peptide (#BLP-GC039).

image 2 :

Peptide (C)KMITTLFDTMRIE corresponding to amino acid residues 206-218 of rat GluD2 (AccessionQ63226). Extracellular N-terminus.

image 3 :

Expression of Glutamate receptor ?2 in rat PC12 cells - Cell surface detection ofGluD2 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells withAnti-GRID2 (extracellular) Antibody(#AGC-039) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B. Cell nuclei (blue) were visualized with Hoechst 33342.

product information

CAT :

AGC-039

SKU :

AGC-039-CF_0.2 ml

Product Name :

Anti-GRID2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63226

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC039

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KMITTLFDTMRIE, corresponding to amino acid residues 206-218 of rat GluD2

Product Page - Scientific background :

Excitatory neurotransmission in the vertebrate central nervous system is mainly mediated by ionotropic glutamate receptors (iGluRs). Molecular cloning identified 18 mammalian iGluR subunits, of which only 16 sort into the traditional pharmacological subfamilies of AMPA, kainate (KA), and N-methyl-D-aspartate (NMDA) receptors. The 2 remaining subunits were termed "orphan" receptors, "glutamate-like" receptors, "nonionotropic" receptors, or, most commonly, delta receptors1.Ionotropic glutamate receptors are integral membrane proteins composed of four large subunits that form a central ion channel pore. Sequence similarity among all known glutamate receptor subunits, including the δ receptors, suggests they share a similar architecture. Glutamate receptor subunits are modular structures that contain four discrete semiautonomous domains: the extracellular amino-terminal domain (ATD), the extracellular ligand-binding domain (LBD), the transmembrane domain (TMD), and an intracellular carboxyl-terminal domain (CTD)2. The delta family of ionotropic glutamate receptors (iGluRs) consists of the glutamate δ1 (GluD1) and glutamate δ2 (GluD2) receptors3. GluD1 is highly expressed in the inner hair cells of the organ of Corti4, diffusely expressed throughout the forebrain during development with high levels in the hippocampus during adulthood3. Deletion of GluD1 leads to a deficit in high frequency hearing in mice5. Genetic association studies have established the GRID1 gene, which codes for GluD1, is a strong candidate gene for schizophrenia, bipolar disorder, and major depressive disorder6. Copy number variation studies have also implicated GRID1 in autism spectrum disorder (ASD)7. In addition, GRID1 gene is localized to the 10q22-q23 genomic region which is a site for recurrent deletions associated with cognitive and behavioral abnormalities8.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Ionotropic glutamate receptor δ2, GluR delta-2 subunit, GluD2

Short Description :

A Rabbit Polyclonal Antibody to Glutamate Receptor δ2 (GRID2)

Long Description :

Anti-GRID2 (extracellular) Antibody (#AGC-039) is a highly specific antibody directed against an epitope of the rat Glutamate receptor δ2. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize GluD2 from rat, mouse, and human samples.

Negative Control :

BLP-GC039

Positive Control :

Synonyms :

Ionotropic glutamate receptor δ2, GluR delta-2 subunit, GluD2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRID2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage