Anti-GRID1 (extracellular) Antibody | Alomone Labs AGC-038 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GRID1 (extracellular) Antibody

catalog :

AGC-038

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Gandhi P, Gawande D, Shelkar G, Gakare S, Kiritoshi T, Ji G, et al. Dysfunction of Glutamate Delta-1 Receptor-Cerebellin 1 Trans-Synaptic Signaling in the Central Amygdala in Chronic Pain. Cells. 2021;10: pubmed publisher
Molica F, Meens M, Dubrot J, Ehrlich A, Roth C, Morel S, et al. Pannexin1 links lymphatic function to lipid metabolism and atherosclerosis. Sci Rep. 2017;7:13706 pubmed publisher
Suryavanshi P, Gupta S, Yadav R, Kesherwani V, Liu J, Dravid S. Glutamate Delta-1 Receptor Regulates Metabotropic Glutamate Receptor 5 Signaling in the Hippocampus. Mol Pharmacol. 2016;90:96-105 pubmed publisher

image

image 1 :

Western blot analysis of human CCF-STTG1 astrocytoma cell lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat brain lysate (lines 3 and 6): - 1-3. Anti-GRID1 (extracellular) Antibody (#AGC-038), (1:200).4-6. Anti-GRID1 (extracellular) Antibody, preincubated with GRID1 (extracellular) Blocking Peptide (#BLP-GC038).

image 2 :

Expression of Glutamate receptor δ1 in rat cortex and medial septum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GRID1 (extracellular) Antibody (#AGC-038), (1:400). A. Staining in cortex. B. Staining in medial septum. In both regions, GluD1 expression (red) is detected in neurons (arrows). DAPI is used as the counterstain (blue).

image 3 :

Expression of Glutamate receptor ?1 in rat cortex and medial septum - Immunohistochemical staining of perfusion-fixed frozen rat brain sectionsusingAnti-GRID1 (extracellular) Antibody(#AGC-038) (1:400). A. Staining in cortex. B. Staining in medial septum. In both regions GluD1 expression (red) is detected in neurons (arrows). DAPI is used as the counterstain (blue).

product information

CAT :

AGC-038

SKU :

AGC-038-CF_0.2 ml

Product Name :

Anti-GRID1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62640

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC038

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KDMRKLATWDSEK, corresponding to amino acid residues 407-419 of rat GluD1

Product Page - Scientific background :

Excitatory neurotransmission in the vertebrate central nervous system is mainly mediated by ionotropic glutamate receptors (iGluRs). Molecular cloning identified 18 mammalian iGluR subunits, of which only 16 sort into the traditional pharmacological subfamilies of AMPA, kainate (KA), and N-methyl-D-aspartate (NMDA) receptors. The 2 remaining subunits were termed "orphan" receptors, "glutamate-like" receptors, "nonionotropic" receptors, or, most commonly, delta receptors1.Ionotropic glutamate receptors are integral membrane proteins composed of four large subunits that form a central ion channel pore. Sequence similarity among all known glutamate receptor subunits, including the δ receptors, suggests they share a similar architecture. Glutamate receptor subunits are modular structures that contain four discrete semiautonomous domains: the extracellular amino-terminal domain (ATD), the extracellular ligand-binding domain (LBD), the transmembrane domain (TMD), and an intracellular carboxyl-terminal domain (CTD)2. The delta family of ionotropic glutamate receptors (iGluRs) consists of the glutamate δ1 (GluD1) and glutamate δ2 (GluD2) receptors3. GluD1 is highly expressed in the inner hair cells of the organ of Corti4, diffusely expressed throughout the forebrain during development with high levels in the hippocampus during adulthood3. Deletion of GluD1 leads to a deficit in high frequency hearing in mice5. Genetic association studies have established the GRID1 gene, which codes for GluD1, is a strong candidate gene for schizophrenia, bipolar disorder, and major depressive disorder6. Copy number variation studies have also implicated GRID1 in autism spectrum disorder (ASD)7. In addition, GRID1 gene is localized to the 10q22-q23 genomic region which is a site for recurrent deletions associated with cognitive and behavioral abnormalities8.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Ionotropic glutamate receptor δ1, GluR delta-1 subunit, GluD1

Short Description :

A Rabbit Polyclonal Antibody to Glutamate Receptor δ1 (GRID1)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat Glutamate receptor δ1. Anti-GRID1 (extracellular) Antibody (#AGC-038) can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize GluD1 from rat, mouse, and human samples.

Negative Control :

BLP-GC038

Positive Control :

Synonyms :

Ionotropic glutamate receptor δ1, GluR delta-1 subunit, GluD1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRID1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage