Anti-VGLUT3 Antibody | Alomone Labs AGC-037 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-VGLUT3 Antibody

catalog :

AGC-037

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Rajaram E, Kaltenbach C, Fischl M, Mrowka L, Alexandrova O, Grothe B, et al. Slow NMDA-Mediated Excitation Accelerates Offset-Response Latencies Generated via a Post-Inhibitory Rebound Mechanism. Eneuro. 2019;6: pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-VGLUT3 Antibody (#AGC-037), (1:500).3,4. Anti-VGLUT3 Antibody, preincubated with VGLUT3 Blocking Peptide (#BLP-GC037).

image 2 :

Expression of VGLUT3 in rat and mouse brain - Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections using Anti-VGLUT3 Antibody (#AGC-037), (1:400), followed by anti-rabbit-Cy3 antibody (red). Nuclei were labeled with DAPI and appear in blue. A. VGLUT3 staining in rat piriform cortex appears as grains along layer 2 (arrows). B. VGLUT3 staining in mouse hippocampal CA3 region appeared in the pyramidal cell body (P and arrows).

image 3 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain membranes: - 12. Anti-VGLUT3 Antibody(#AGC-037) (1:500).34. Anti-VGLUT3 Antibody preincubated with the negative control antigen.

product information

CAT :

AGC-037

SKU :

AGC-037-CF_0.2 ml

Product Name :

Anti-VGLUT3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7TSF2

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC037

Homology :

Mouse - 12/13 amino acid residues identical; human - 10/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ELNHEAFVSPRKK, corresponding to amino acid residues 533-545 of rat VGLUT3

Product Page - Scientific background :

Central nervous system neurons have traditionally been thought to express exclusively membrane transporters and/or vesicular transporters for their transmitter. Three vesicular glutamate transporters (VGLUTs) have recently been cloned: BNPI/VGLUT1 (a brain-specific sodium dependent inorganic phosphate (Pi) transporter), and its homologs DNPI/VGLUT2 (differentiation-associated sodium-dependent Pi transporter) and VGLUT31. These transporters mediate glutamate uptake inside presynaptic vesicles and are anatomical and functional markers of glutamatergic excitatory transmission2.VGLUT 1-3 are very similar in structure and function, but are used by different neuronal populations. VGLUT3 is a glycosylated transmembrane protein with ten putative transmembrane domains (TMD I-X) and both amino- and carboxyl-termini located in the cytosol3.VGLUT3 is localized in a limited number of glutamatergic neurons in multiple brain regions: neocortex, hippocampus, olfactory bulb, hypothalamus, substania nigra and raphe nuclei. Additionally, VGLUT3 is expressed in a population of symmetrical synapses. It is detected in hippocampal and cortical GABAergic neurons, cholinergic neurons in the striatum and serotonergic neurons in the raphe nuclei4.Mice lacking VGLUT3 are congenitally deaf due to loss of glutamate release at the inner hair cell afferent synapse5. In addition loss of VGLUT3 specifically impairs mechanical pain sensation6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Vesicular glutamate transporter 3, SLC17A8

Short Description :

A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Vesicular glutamate transporter 3. Anti-VGLUT3 Antibody (#AGC-037) can be used in western blot and immunohistochemistry applications. It has been designed to recognize VGLUT3 from rat, mouse and human samples.

Negative Control :

BLP-GC037

Positive Control :

Synonyms :

Vesicular glutamate transporter 3, SLC17A8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC17A8

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage