Guinea Pig Anti-VGLUT2 Antibody | Alomone Labs AGC-036-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea Pig Anti-VGLUT2 Antibody

catalog :

AGC-036-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Guinea Pig Anti-VGLUT2 Antibody (#AGC-036-GP), (1:200).3-4. Guinea Pig Anti-VGLUT2 Antibody, preincubated with VGLUT2 Blocking Peptide (BLP-GC036).

image 2 :

Expression of VGLUT2 in rat parietal cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-VGLUT2 Antibody (#AGC-036-GP), (1:200), followed by goat anti-Guinea Pig-AlexaFluor-594. A. VGLUT2 immunoreactivity (red) appears in pyramidal neuronal profiles (horizontal arrows). B. Pre-incubation of the antibody with VGLUT2 Blocking Peptide (BLP-GC036), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGC-036-GP

SKU :

AGC-036-GP-CF_0.2 ml

Product Name :

Guinea Pig Anti-VGLUT2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JI12

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-GC036

Homology :

Mouse - identical; human - 11 out of 12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDGKPLEVPEKK, corresponding to amino acid residues 45-56 of rat VGLUT2

Product Page - Scientific background :

Central nervous system neurons have traditionally been thought to express exclusively membrane transporters and/or vesicular transporters for their transmitter. Three vesicular glutamate transporters (VGLUTs) have been cloned: BNPI/VGLUT1 (a brain-specific sodium dependent inorganic phosphate (Pi) transporter), and its homologs DNPI/VGLUT2 (differentiation-associated sodium-dependent Pi transporter) and VGLUT31. These transporters mediate glutamate uptake inside presynaptic vesicles and are anatomical and functional markers of glutamatergic excitatory transmission2.BNPI/DNPI encodes a membrane protein with 6-8 putative transmembrane domains which exhibits weak similarities to a kidney Na+-dependent inorganic phosphate co-transporter3. The transporters use a membrane potential gradient set by the vesicular H+-ATPase for glutamate uptake4. VGLUT1-3 are very similar in structure and function, but are used by different neuronal populations. VGLUT1 and VGLUT2 are expressed by the cortical and subcortical neurons respectively. VGLUT3 is expressed by non-glutamatergic neurons5. VGLUT2 is expressed in the thalamus, brainstem, and deep cerebellar nuclei2. A Recent study has shown that targeted deletion of VGLUT2 in mice causes perinatal lethality and a 95% reduction in evoked glutamatergic responses in thalamic neurons, although hippocampal synapses function normally. Behavioral analysis of heterozygous VGLUT2 mice showed discrete behavioral phenotypes that suggest a deficit in thalamic processing6.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Vesicular glutamate transporter 2, Differentiation-associated Na+-dependent inorganic phosphate cotransporter, DNPI, SLC17A6

Short Description :

A Guinea Pig Polyclonal Antibody to Vesicular Glutamate Transporter 2

Long Description :

Guinea Pig Anti-VGLUT2 Antibody (#AGC-036-GP, formerly AGP-131) is a highly specific antibody directed against an epitope of rat Vesicular glutamate transporter 2. The antibody can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize VGLUT2 from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-VGLUT2 Antibody (#AGC-036) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Negative Control :

BLP-GC036

Positive Control :

Synonyms :

Vesicular glutamate transporter 2, Differentiation-associated Na+-dependent inorganic phosphate cotransporter, DNPI, SLC17A6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC17A6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage