Anti-VGLUT1 Antibody | Alomone Labs AGC-035 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-VGLUT1 Antibody

catalog :

AGC-035

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Coviello S, Gramuntell Y, Klimczak P, Varea E, Blasco Iba xf1 ez J, Crespo C, et al. Phenotype and Distribution of Immature Neurons in the Human Cerebral Cortex Layer II. Front Neuroanat. 2022;16:851432 pubmed publisher
Pernice H, Schieweck R, Jafari M, Straub T, Bilban M, Kiebler M, et al. Altered Glutamate Receptor Ionotropic Delta Subunit 2 Expression in Stau2-Deficient Cerebellar Purkinje Cells in the Adult Brain. Int J Mol Sci. 2019;20: pubmed publisher
Qiu M, Li J, Tan L, Zhang M, Zhou G, Zeng T, et al. Targeted Ablation of Distal Cerebrospinal Fluid-Contacting Nucleus Alleviates Renal Fibrosis in Chronic Kidney Disease. Front Physiol. 2018;9:1640 pubmed publisher

image

image 1 :

Western blot analysis of rat cortex lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1,2. Anti-VGLUT1 Antibody (#AGC-035), (1:200).3,4. Anti-VGLUT1 Antibody, preincubated with VGLUT1 Blocking Peptide (#BLP-GC035).

image 2 :

Expression of VGLUT1 in rat cortex - Immunohistochemical staining of perfusion-fixed rat brain frozen sections using Anti-VGLUT1 Antibody (#AGC-035), (1:400), followed by goat-anti-rabbit-Alexa-488. VGLUT1 staining (green) appears in neurons of the pyramidal layer of the parietal cortex (arrows). DAPI is used as the nuclear counterstain (blue).

product information

CAT :

AGC-035

SKU :

AGC-035-CF_0.2 ml

Product Name :

Anti-VGLUT1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62634

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC035

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFRQEEFRKLAGR, corresponding to amino acid residues 2-14 of rat VGLUT1

Product Page - Scientific background :

Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system (CNS) and is responsible for most of the fast synaptic neurotransmission. Glutamate has great contribution for learning, memory, and neuronal plasticity. Dysregulation of glutamate transmission plays a role in the pathophysiology of several psychiatric and neurological diseases1. Three vesicular glutamate transporters (VGLUT1, VGLUT2, and VGLUT3) have been identified. They are responsible for packaging and transporting glutamate into synaptic vesicles. VGLUTs in part regulate the storage and release of glutamate in excitatory circuits in the brain.All three VGLUT isoforms are highly conserved and have the same predicted topology including 12 putative transmembrane domains. They all share a conserved dileucine-like motif in the C terminus. This motif was found to be important for VGLUT1 endocytosis. In addition, VGLUT1 contains two polyproline domains distal to the C-terminal dileucine-like motif, including one that interacts with the endocytic BAR (Bin/Amphiphysin/Rvs) domain protein endophilin. Two additional dileucine-like motifs are present in the N terminus of VGLUT1 that are not well conserved in the other isoforms1-3. VGLUT1 is expressed in the hippocampus, cerebral cortex, and cerebellar cortex and appears to be the main isoform expressed in the cortex. The transporter is also detected in the enteric nervous system and pancreatic tissue. VGLUT1 has been suggested to influence the properties of the release of glutamate1,2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Vesicular glutamate transporter 1, Brain-specific Na+-dependent inorganic phosphate cotransporter, BNPI, SLC17A7

Short Description :

A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 1

Long Description :

Anti-VGLUT1 Antibody (#AGC-035) is a highly specific antibody directed against an epitope of rat Vesicular glutamate transporter 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize VGLUT1 from rat, mouse, and human samples.

Negative Control :

BLP-GC035

Positive Control :

Synonyms :

Vesicular glutamate transporter 1, Brain-specific Na+-dependent inorganic phosphate cotransporter, BNPI, SLC17A7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC17A7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage