Anti-mGluR8 (extracellular) Antibody | Alomone Labs AGC-028 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-mGluR8 (extracellular) Antibody

catalog :

AGC-028

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6): - 1-3. Anti-mGluR8 (extracellular) Antibody (#AGC-028), (1:400).4-6. Anti-mGluR8 (extracellular) Antibody, preincubated with mGluR8 (extracellular) Blocking Peptide (#BLP-GC028).

image 2 :

Expression of mGluR8 in rat globus pallidus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-mGluR8 (extracellular) Antibody (#AGC-028), (1:100). A. Staining reveals expression of mGluR8 (red) in globus pallidus neurons (arrow) and their dendrites. B. The same section was also stained with DAPI (blue) revealing cell nuclei. C. Merge of the two images.

image 3 :

Expression of mGluR8 in rat globus pallidus - Immunohistochemical staining of immersion-fixed free floating rat brain frozen sections usingAnti-mGluR8 (extracellular)Antibody (#AGC-028) (1:100). A. Staining reveals expression of mGluR8 (red) in globus pallidus neurons (arrow) and their dendrites. B. The same section was also stained with DAPI (blue) revealing cell nuclei. C. Merge of the two images.

product information

CAT :

AGC-028

SKU :

AGC-028-CF_0.2 ml

Product Name :

Anti-mGluR8 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70579

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC028

Homology :

Rat, mouse, human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ENFG(S)KLGSHGKR, corresponding to amino acid residues 365-377 of rat mGluR8

Product Page - Scientific background :

Metabotropic glutamate receptors (mGluRs), heptahelical transmembrane receptors coupled to G-proteins, contribute to the regulation of neuronal excitability and synaptic transmission1. Genes coding for eight mGluRs (denoted mGluR1-8) with different splice variants have been identified and classified into three groups based on sequence homology, pharmacology, and signaling pathways. Group I mGluRs (mGluR1 and mGluR5) are primarily located postsynaptically. Group II (mGluR2 and mGluR3) are present both postsynaptically and presynaptically outside the release site. Group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) couple to Gαi/o to inhibit adenylyl cyclase found in the presynaptic active zone2.The mGluR8 receptor is detected in the olfactory bulb, thalamus, pontine gray, cerebral cortex, hippocampus, cerebellum, and retina3. Electrophysiological studies suggest that mGluR8 functions as a presynaptic autoreceptor to regulate glutamate release from the lateral perforant path terminals in the mouse dentate gyrus4. It is suggested that mGluR8, like other group III mGluRs, controls glutamate release by inhibiting voltage-gated Ca2+ channels.Targeted deletion of the mGluR8 gene in mice produces subtle behavioral alterations such as reduced habituation to novelty, hyperactivity, context-dependent but not cue-dependent disruption of the freezing response in the fear conditioning test, and increased anxiety5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H

Short Description :

A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 8

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR8. Anti-mGluR8 (extracellular) Antibody (#AGC-028) can be used in western blot and immunohistochemistry applications. It has been designed to recognize mGluR8 from rat, mouse and human samples.

Negative Control :

BLP-GC028

Positive Control :

Synonyms :

Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRM8

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage