Anti-mGluR6 (extracellular) Antibody | Alomone Labs AGC-026 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-mGluR6 (extracellular) Antibody

catalog :

AGC-026

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Liu D, Song A, Qi X, van Vliet P, Xiao J, Xiong F, et al. Cohesin-protein Shugoshin-1 controls cardiac automaticity via HCN4 pacemaker channel. Nat Commun. 2021;12:2551 pubmed publisher
Akiba R, Matsuyama T, Tu H, Hashiguchi T, Sho J, Yamamoto S, et al. Quantitative and Qualitative Evaluation of Photoreceptor Synapses in Developing, Degenerating and Regenerating Retinas. Front Cell Neurosci. 2019;13:16 pubmed publisher
Goniotaki D, Lakkaraju A, Shrivastava A, Bakirci P, Sorce S, Senatore A, et al. Inhibition of group-I metabotropic glutamate receptors protects against prion toxicity. PLoS Pathog. 2017;13:e1006733 pubmed publisher

image

image 1 :

Western blot analysis of rat eye lysates: - 1. Anti-mGluR6 (extracellular) Antibody (#AGC-026), (1:200).2. Anti-mGluR6 (extracellular) Antibody, preincubated with mGluR6 (extracellular) Blocking Peptide (#BLP-GC026).

image 2 :

Peptide(C)GQSDDSTRK(S)TGEE corresponding to amino acid residues 368-381 of rat mGluR6 (AccessionP35349). Extracellular N-terminus.

image 3 :

Cell surface detection of GLP1R inlive intact human THP-1 monocytic leukemia cells: - ___Cells.___Cells + rabbit IgG isotype control-FITC.___Cells + Anti-GLP1R (extracellular)-FITC Antibody (#AGR-021-F) (5 g).

product information

CAT :

AGC-026

SKU :

AGC-026-CF_0.2 ml

Product Name :

Anti-mGluR6 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35349

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC026

Homology :

Mouse, rat - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GQSDDSTRK(S)TGEE, corresponding to amino acid residues 368-381 of rat mGluR6

Product Page - Scientific background :

Glutamate is the principal excitatory neurotransmitter in the central nervous system. It operates through two main classes of receptors: ionotropic receptors (iGluRs), which are ligand-gated ion channels, and metabotropic receptors (mGluRs), which couple to G-proteins to modulate cellular responses via ion channels or intracellular second messengers.The metabotropic receptors include eight family members (mGluR1-mGluR8) classified into three groups according to their sequence similarities, signal transduction, and agonist selectivity. They all share a common structure, being composed of a single polypeptide with large N-terminal extracellular domain connected through a seven transmembrane domain motif to the intracellular C-terminal tail1,2. The different mGluR subtypes are localized at both presynaptic and postsynaptic membranes, and are involved in the generation of slow excitatory and inhibitory synaptic potentials, modulation of synaptic transmission, synaptic integration, and plasticity3.mGluR6 belongs to the third group of metabotrophic glutamate receptors. Its expression in the central nervous system is restricted to retinal rod bipolar cells, and it's localized to their postsynaptic dendritic membranes3,4. In the peripheral nervous system, it is also detected in bone marrow stromal cells6. mGluR6 inhibits adenylate cyclase, and was shown to inhibit Ca2+ influx and nitric oxide synthase activity3,5,6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Metabotropic glutamate receptor 6, GRM6, mGlu6, Gprc1f

Short Description :

A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 6

Long Description :

Anti-mGluR6 (extracellular) Antibody (#AGC-026) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially detect the receptor in living cells. It has been designed to recognize mGluR6 from rat, mouse, and human samples.

Negative Control :

BLP-GC026

Positive Control :

Synonyms :

Metabotropic glutamate receptor 6, GRM6, mGlu6, Gprc1f

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRM6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage