Anti-SLC1A7 (extracellular) Antibody | Alomone Labs AGC-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC1A7 (extracellular) Antibody

catalog :

AGC-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate: - 1,2. Anti-SLC1A7 (extracellular) Antibody (#AGC-025), (1:200).3,4. Anti-SLC1A7 (extracellular) Antibody, preincubated with SLC1A7 (extracellular) Blocking Peptide (#BLP-GC025).

image 2 :

Peptide (C)KYLYEDEG(S)WTRNS corresponding to amino acid residues 288-301 of rat GLP1R (AccessionP32301). 2nd extracellular loop.

product information

CAT :

AGC-025

SKU :

AGC-025-CF_0.2 ml

Product Name :

Anti-SLC1A7 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8JZR4

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC025

Homology :

Rat - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KETTEQSGKPVMS, corresponding to amino acid residues 122-134 of mouse EAAT5

Product Page - Scientific background :

Glutamate transporters play important roles in the termination of excitatory neurotransmission and in providing cells throughout the body with glutamate for metabolic purposes.SLC1A7 (EAAT5) belongs to The SLC1 protein family which contains a range of human and prokaryotic glutamate and aspartate transporters1. EAAT5 is expressed mainly in photoreceptors and bipolar cells in the retina and is essential for the maintenance of normal excitatory synaptic transmission as it determines the time course of glutamate receptor activation. EAAT5 consists of 3 identical subunits that are functionally coupled to the sodium (Na+), potassium (K+)-ATPase as part of the same macromolecular complex. The transporter operates via an "alternate access mechanism" whereby the translocation of one molecule of glutamate into the cell is coupled with the inward cotransport with 3Na+ and 1H+ ions. In turn, the return of the empty carrier to the extracellular side of the plasma membrane is coupled to the export of 1K+ ion. In addition, EAAT5, may function as glutamate-gated chloride (Cl−) channel, generating an anion current that is not stoichiometrically coupled to the uptake of glutamate2.EAAT5 has been linked to the development of hippocampal sclerosis. Hippocampal sclerosis of aging (HS-Aging) is a causative factor in a large proportion of elderly dementia cases3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Excitatory amino acid transporter 5, EAAT5, Retinal glutamate transporter

Short Description :

A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 5 (SLC1A7)

Long Description :

Anti-SLC1A7 (extracellular) Antibody (#AGC-025) is a highly selective antibody directed against an epitope of mouse Excitatory amino acid transporter 5. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize EAAT5 from mouse, human, and rat samples.

Negative Control :

BLP-GC025

Positive Control :

Synonyms :

Excitatory amino acid transporter 5, EAAT5, Retinal glutamate transporter

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC1A7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage